Koupai-Abyazani M R, Esaw B, Laviolette B
Can Test Laboratories Ltd., Vancouver, British Columbia, Canada.
J Anal Toxicol. 1997 Jul-Aug;21(4):301-5. doi: 10.1093/jat/21.4.301.
Urine and serum samples collected from four standard-bred mares after and oral regimen administration of methocarbamol were extracted and analyzed. The method consisted of enzyme hydrolysis followed by a one-step liquid-liquid extraction, separation on a reversed-phase (RP-18) column, and detection using an ultraviolet (UV) detector. The confirmation was carried out using a liquid chromatography-atmospheric pressure ionization-mass spectrometry (LC-API-MS) system. Maximum methocarbamol concentrations of 1498, 1734, 1547, 2322 micrograms/mL in urine and 4.9, 1.7, and 3.6 micrograms/mL in serum were observed. The peak concentrations of the drug were detected 1-4 h (urine) and 10-60 min (serum) after administration to four horses. The method validation results and drug elimination profiles for both urine and serum are presented and discussed.
从四匹标准品种母马口服美索巴莫后采集尿液和血清样本，进行提取和分析。该方法包括酶水解，然后进行一步液液萃取，在反相（RP - 18）柱上分离，并使用紫外（UV）检测器进行检测。使用液相色谱 - 大气压电离 - 质谱（LC - API - MS）系统进行确证。观察到尿液中美索巴莫的最大浓度为1498、1734、1547、2322微克/毫升，血清中为4.9、1.7和3.6微克/毫升。给四匹马给药后，在1 - 4小时（尿液）和10 - 60分钟（血清）检测到药物的峰值浓度。展示并讨论了尿液和血清的方法验证结果以及药物消除曲线。
