Maayan M L, Volpert E M, Dawry F P
J Endocrinol Invest. 1978 Oct;1(4):299-304. doi: 10.1007/BF03350973.
LATS containing sera and a number of Graves' disease sera stimulated T4 release from mouse thyroids in vitro as determined by RIA, thus confirming the presence of a thyroid hormone releasing factor in sera of thyrotoxic patients. The pattern of stimulation was similar to that previously shown for TSH in terms of T4 release time sequence. cAMP increase and catecholamine inhibition via alpha-adrenergic receptors. In the same in vitro system, neutralization with a human thyroid homogenate showed presence of LATS-Protector (LPA) in LATS negative thyrotoxic sera. The present study describes a simpler procedure for estimating LATS or similar activity, as compared to the McKenzie assay, and suggests identical receptor sites for TSH and other thyroid stimulators.
通过放射免疫分析法测定，含有长效甲状腺刺激素（LATS）的血清以及一些格雷夫斯病血清在体外刺激小鼠甲状腺释放甲状腺素（T4），从而证实甲状腺毒症患者血清中存在甲状腺激素释放因子。就T4释放时间顺序而言，刺激模式与先前TSH的刺激模式相似。环磷酸腺苷（cAMP）增加以及通过α-肾上腺素能受体的儿茶酚胺抑制作用。在同一体外系统中，用人甲状腺匀浆进行中和反应显示，LATS阴性的甲状腺毒症血清中存在LATS保护因子（LPA）。与麦肯齐试验相比，本研究描述了一种更简单的估计LATS或类似活性的方法，并提示TSH和其他甲状腺刺激物具有相同的受体位点。
