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Production and secretion of Aspergillus nidulans catalase B in filamentous fungi driven by the promoter and signal peptide of the Cladosporium fulvum hydrophobin gene hcf-1.

作者信息

Johnson Hannah, Whiteford James R, Eckert Sabine E, Spanu Pietro D

机构信息

Department of Biological Sciences, Imperial College London, Sir Alexander Fleming Building, Imperial College Road, London SW7 2AZ, UK.

出版信息

Curr Genet. 2003 Nov;44(3):155-63. doi: 10.1007/s00294-003-0421-4. Epub 2003 Sep 4.

DOI:10.1007/s00294-003-0421-4
PMID:12955453
Abstract

We describe here the use of sequences from the hydrophobin gene hcf-1 of Cladosporium fulvum to construct pCatBex, a vector for high-level expression and secretion of CatB, a catalase from Aspergillus nidulans. Transformation of C. fulvum with pCatBex results in a 60-fold increase in the mycelial activity in the fungus and the appearance of up to 5.4 mkat/l of catalase in the growth medium. The levels of catalase in the supernatant increased dramatically following removal of nitrogen from the medium. Conversely, the overall specific activity of catalase in the cytoplasm did not change appreciably. This indicates that nitrogen depletion induces greater secretion of protein. The vector pCatBex also directs the expression and secretion of CatB in Magnaporthe grisea and may be a useful vector for the expression of genes in other filamentous fungi.

摘要

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本文引用的文献

1
Hydrophobins and the interactions between fungi and plants.疏水性蛋白和真菌与植物之间的相互作用。
Mol Plant Pathol. 2002 Sep 1;3(5):391-400. doi: 10.1046/j.1364-3703.2002.00129.x.
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Filamentous fungi as cell factories for heterologous protein production.丝状真菌作为用于异源蛋白生产的细胞工厂。
Trends Biotechnol. 2002 May;20(5):200-6. doi: 10.1016/s0167-7799(02)01933-9.
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Hydrophobins: multipurpose proteins.疏水蛋白：多功能蛋白质。
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The hydrophobin HCf-1 of Cladosporium fulvum is required for efficient water-mediated dispersal of conidia.番茄叶霉病菌的疏水蛋白HCf-1是分生孢子通过水进行有效传播所必需的。
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Fungal Genet Biol. 2001 Feb;32(1):55-65. doi: 10.1006/fgbi.2000.1245.
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Isolation and characterisation of five different hydrophobin-encoding cDNAs from the fungal tomato pathogen Cladosporium fulvum.从番茄真菌病原体fulvum中分离和鉴定五个不同的疏水蛋白编码cDNA
Mol Gen Genet. 1999 Jun;261(4-5):644-52. doi: 10.1007/s004380050007.
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Co-suppression of the hydrophobin gene HCf-1 is correlated with antisense RNA biosynthesis in Cladosporium fulvum.
Mol Gen Genet. 1998 Oct;259(6):630-8. doi: 10.1007/s004380050857.
9
HCF-1, a hydrophobin from the tomato pathogen Cladosporium fulvum.HCF-1，一种来自番茄病原菌fulvum的疏水蛋白。 （注：这里原文中“Cladosporium fulvum”可能有误，常见的番茄病原菌是“Cladosporium fulvum”的正确中文名是“番茄叶霉病菌” ，但按要求未做修改翻译。 ）
Gene. 1997 Jul 1;193(1):89-96. doi: 10.1016/s0378-1119(97)00090-5.
10
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites.原核生物和真核生物信号肽的鉴定及其切割位点的预测。
Protein Eng. 1997 Jan;10(1):1-6. doi: 10.1093/protein/10.1.1.