Suller M T, Stark J M, Lloyd D
School of Pure and Applied Biology, University of Wales College of Cardiff, UK.
J Antimicrob Chemother. 1997 Jul;40(1):77-83. doi: 10.1093/jac/40.1.77.
Flow cytometry using the anionic membrane potential-sensitive fluorescent probe, bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)), enabled assessment of antibiotic-induced membrane perturbation in five clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and two antibiotic-sensitive reference strains, NCTC 6571 and 8325-4, after establishment of steady-state growth in liquid cultures inoculated from single colonies. Flow cytometric indications of the enhanced DiBAC4(3) uptake after treatment with vancomycin at 0.1, 1, 4 and 10 x MIC showed excellent comparison with viability losses quantified as cfu on solid agar in MRSA isolate QC. The antibiotic susceptibility patterns to benzylpenicillin, methicillin and vancomycin for all isolates used in this study could be determined in 2-4 h from an overnight plate culture. This technique thus provides a rapid and reproducible antibiotic sensitivity test which may be applicable in routine clinical practice.
使用阴离子膜电位敏感荧光探针双（1,3 - 二丁基巴比妥酸）三甲川草酚菁（DiBAC4(3)）的流式细胞术，在从单菌落接种的液体培养物中建立稳态生长后，能够评估五种耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株以及两种抗生素敏感参考菌株NCTC 6571和8325 - 4中抗生素诱导的膜扰动。在MRSA分离株QC中，用0.1、1、4和10倍最低抑菌浓度（MIC）的万古霉素处理后，DiBAC4(3)摄取增强的流式细胞术指示与在固体琼脂上以菌落形成单位（cfu）量化的活力损失显示出极好的相关性。本研究中使用的所有分离株对苄青霉素、甲氧西林和万古霉素的抗生素敏感性模式可在过夜平板培养后2 - 4小时内确定。因此，该技术提供了一种快速且可重复的抗生素敏感性测试，可能适用于常规临床实践。
