Grove G L
J Histochem Cytochem. 1979 Oct;27(10):1375-6. doi: 10.1177/27.10.92495.
During recent years, the utilization of microspectrophotometry in biomedical research has increased at a rapid pace. This growth has been facilitated by both the advent of commercially available instruments which are reliable and manageable as well as improvements in histochemical techniques. This paper discusses the fundamental principles of visible light microspectrophotometry and important design criteria as well as briefly reviewing a wide range of applications. Special emphasis will be given to the Vickers M85 scanning integrating microspectrophotometer which is being routinely employed for both clinical and laboratory studies by a sizeable number of investigators.
近年来，显微分光光度法在生物医学研究中的应用迅速增加。可靠且易于操作的商用仪器的出现以及组织化学技术的改进推动了这种增长。本文讨论了可见光显微分光光度法的基本原理和重要的设计标准，并简要回顾了广泛的应用。将特别强调维氏M85扫描积分显微分光光度计，相当多的研究人员经常将其用于临床和实验室研究。
