Ferguson M A
Department of Resources, Wildlife and Economic Development, Government of the Northwest Territories, Canada.
J Wildl Dis. 1997 Jul;33(3):536-43. doi: 10.7589/0090-3558-33.3.536.
The standard tube agglutination test (STAT) and the complement fixation test (CFT) were used to assess the seroprevalence of antibodies to Brucella spp. in caribou (Rangifer tarandus) from three populations on Baffin Island, Canada. During late winter from 1983 to 1986, sera from 17 of 40 North Baffin (43%), 11 of 33 Northeast Baffin (33%) and 12 of 82 South Baffin (15%) adult caribou had antibodies in the STAT at 1:50 or the CFT at 1:5. Seroprevalence increased as caribou matured with one (4%) of 25 calves, four (13%) of 31 yearlings, and 40 (26%) of 155 adult caribou being positive. However, seroprevalence did not differ with sex in any age class. Positive antibody titers were higher in adult females sampled in May, 3 to 4 wk before parturition, than in adult females sampled in late March and April. The strength of positive titers did not differ with the time of sampling among adult males. Pathologic signs of brucellosis were found in three (13%) of 23 caribou that were assumed to have active infections (caribou with CFT titers > 1:160). Brucella suis biovar 4 was isolated from 24 (60%) of 40 caribou from which lesions were submitted. Between 1986 and 1990, the annual incidence of reported human (Homo sapiens) cases averaged 3.4 (34:100,000) on Baffin Island.
采用标准试管凝集试验（STAT）和补体结合试验（CFT）评估加拿大巴芬岛三个种群的北美驯鹿（Rangifer tarandus）中布鲁氏菌属抗体的血清阳性率。在1983年至1986年冬末，40头北巴芬成年驯鹿中有17头（43%）、33头东北巴芬成年驯鹿中有11头（33%）以及82头南巴芬成年驯鹿中有12头（15%）的血清在STAT中呈现1:50的抗体或在CFT中呈现1:5的抗体。血清阳性率随着驯鹿成熟而增加，25头幼鹿中有1头（4%）、31头一岁龄鹿中有4头（13%）以及155头成年驯鹿中有40头（26%）呈阳性。然而，在任何年龄组中，血清阳性率在性别上并无差异。五月采集的成年雌性驯鹿（分娩前3至4周）的阳性抗体滴度高于三月下旬和四月采集的成年雌性驯鹿。成年雄性驯鹿中，阳性滴度的强度在采样时间上并无差异。在23头被认为有活跃感染的驯鹿（CFT滴度>1:160的驯鹿）中有3头（13%）发现了布鲁氏菌病的病理迹象。从提交病变样本的40头驯鹿中的24头（60%）分离出猪布鲁氏菌生物变种4。1986年至1990年间，巴芬岛上报的人类（智人）病例年平均发病率为3.4（34:100,000）。
