Forbes L B, Tessaro S V
Health of Animals Laboratory, Food Production and Inspection Branch, Agriculture Canada, University of Saskatchewan Campus, Saskatoon.
J Am Vet Med Assoc. 1993 Jul 15;203(2):289-94.
Sixteen reindeer (Rangifer tarandus tarandus) naturally infected with Brucella suis biovar 4 were penned with 6 male and 2 female cattle for 30 days, then removed and euthanatized. During this period, 5 reindeer had fawns, and 2 reindeer aborted. Brucella suis biovar 4 was recovered from all adult reindeer at necropsy. Nine reindeer had B suis biovar 4 in uterus, udder, and/or milk. The cattle were euthanatized 2 months after the reindeer were removed. Clinical or pathologic signs of disease were not observed in the cattle. Brucella suis biovar 4 was isolated from 2 male and from both female cattle at necropsy. The female cattle had positive reactions on the buffered plate antigen test, brucellosis card test, tube agglutination test, complement fixation test, and indirect enzyme immunoassay for most of the experiment, but the males had inconsistent reactions on these tests. The indirect enzyme immunoassay was the only test to detect all cattle from which bacteria were cultured. This study revealed that caution is warranted before moving reindeer or caribou into areas of traditional agriculture.
将16头自然感染猪布鲁氏菌生物变种4的驯鹿（Rangifer tarandus tarandus）与6头公牛和2头母牛圈养30天，然后移出并实施安乐死。在此期间，5头驯鹿产仔，2头驯鹿流产。尸检时从所有成年驯鹿中分离出猪布鲁氏菌生物变种4。9头驯鹿的子宫、乳房和/或乳汁中含有猪布鲁氏菌生物变种4。驯鹿移出2个月后，对牛实施安乐死。未观察到牛出现疾病的临床或病理症状。尸检时从2头公牛和2头母牛中分离出猪布鲁氏菌生物变种4。在实验的大部分时间里，母牛在缓冲平板抗原试验、布鲁氏菌病卡片试验、试管凝集试验、补体结合试验和间接酶免疫测定中呈阳性反应，但公牛在这些试验中的反应不一致。间接酶免疫测定是唯一能检测出所有培养出细菌的牛的试验。这项研究表明，在将驯鹿或北美驯鹿转移到传统农业地区之前，必须谨慎行事。
