Martinello F, Galuppo F, Ostanello F, Guberti V, Prosperi S
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università di Bologna, Italy.
J Wildl Dis. 1997 Jul;33(3):628-31. doi: 10.7589/0090-3558-33.3.628.
One hundred fifteen samples of wolf (Canis lupus) feces were collected during 1994 to 1995 from four free-living populations of the north central Apennines Mountains, Italy. The samples were tested for canine parvovirus by antigen-capture enzyme-linked immunosorbent assay (ELISA), hemagglutination, and virus isolation. Four of these samples were positive by virus isolation as confirmed by electron microscopy. All positive samples were from Casentino Park in Tuscany. This is the first definitive observation of canine parvovirus in wolves from Europe.
1994年至1995年期间,从意大利亚平宁山脉中北部四个自由生活的狼群中采集了115份狼(Canis lupus)粪便样本。通过抗原捕获酶联免疫吸附测定(ELISA)、血凝试验和病毒分离对样本进行犬细小病毒检测。其中4份样本经病毒分离呈阳性,电子显微镜检查予以证实。所有阳性样本均来自托斯卡纳的卡森蒂诺公园。这是欧洲狼感染犬细小病毒的首次确切观察。