Baggia S, Lyons J L, Angell E, Barkhuizen A, Han Y B, Planck S R, Taurog J D, Rosenbaum J T
Department of Ophthalmology, Oregon Health Sciences University, Portland, USA.
J Investig Med. 1997 Jun;45(5):295-301.
Humans with the major histocompatibility antigen B27 (HLA-B27) are especially at risk for developing rheumatic disorders such as ankylosing spondylitis and Reiter's syndrome. Acute anterior uveitis (AAU) often occurs in association with these diseases or in HLA B27 positive individuals without joint disease.
We induced acute anterior uveitis in Lewis rats by a standard model, the intraperitoneal injection of 200 micrograms of Escherichia coli endotoxin. We also developed a novel model of uveitis secondary to gram-negative infection.
Transgenic rats that expressed a low copy number of the B27 gene did not differ statistically from litter mate controls in the intensity of anterior uveitis as judged by histology, enumeration of cells in aqueous humor, protein in aqueous humor, or slit lamp examination. The majority of rats exposed to live Salmonella enteritidis or Yersinia enterocolitica 0:3 using either an oral or intravenous route of infection developed anterior uveitis. In contrast to the disease induced by endotoxin that is most intense 24 hours after the endotoxin challenge, uveitis induced by live bacteria usually began 7 to 9 days after exposure to bacterial products, was more often unilateral, persisted for as long as 3 weeks, and was sometimes recurrent. The expression of HLA-B27 did not appear to influence the incidence or severity of uveitis in B27+ low copy heterozygous animals.
This rat model of AAU should facilitate evaluation of bacterial antigenic component(s) involved in the pathogenesis of live gram-negative bacteria induced AAU.
携带主要组织相容性抗原B27（HLA - B27）的人类尤其易患风湿性疾病，如强直性脊柱炎和赖特综合征。急性前葡萄膜炎（AAU）常与这些疾病相关，或发生于无关节疾病的HLA B27阳性个体。
我们通过标准模型，即腹腔注射200微克大肠杆菌内毒素，在刘易斯大鼠中诱导急性前葡萄膜炎。我们还建立了一种继发于革兰氏阴性菌感染的葡萄膜炎新模型。
通过组织学、房水中细胞计数、房水中蛋白质含量或裂隙灯检查判断，表达低拷贝数B27基因的转基因大鼠在前葡萄膜炎强度方面与同窝对照大鼠无统计学差异。大多数经口服或静脉途径感染活肠炎沙门氏菌或小肠结肠炎耶尔森氏菌0:3的大鼠发生了前葡萄膜炎。与内毒素诱导的疾病在毒素攻击后24小时最为严重不同，活细菌诱导的葡萄膜炎通常在接触细菌产物后7至9天开始，更常为单侧性，持续长达3周，且有时会复发。HLA - B27的表达似乎不影响B27 +低拷贝杂合动物葡萄膜炎的发病率或严重程度。
这种AAU大鼠模型应有助于评估参与活革兰氏阴性菌诱导的AAU发病机制的细菌抗原成分。
