Huhtinen M, Laasila K, Granfors K, Puolakkainen M, Seppälä I, Laasonen L, Repo H, Karma A, Leirisalo-Repo M
Department of Ophthalmology, University of Helsinki, Finland.
Ann Rheum Dis. 2002 Nov;61(11):1012-6. doi: 10.1136/ard.61.11.1012.
To study the infectious background of patients with a history of acute anterior uveitis (AAU) and healthy control subjects.
Sixty four patients with previous AAU and 64 sex and age matched controls were studied. Serum antibodies to Salmonellae, Yersiniae, Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, Campylobacter jejuni, and Borrelia burgdorferi were measured using enzyme linked immunosorbent assay (ELISA), and antibodies to Chlamydia trachomatis and Chlamydia pneumoniae by microimmunofluorescence test. Peripheral blood mononuclear cells (PBMCs), separated by density gradient centrifugation, were studied for Salmonella and Yersinia antigens by means of an immunofluorescence test, and for C pneumoniae DNA with a polymerase chain reaction (PCR).
Neither prevalence nor levels of single microbial antibodies studied differed between the patients and control subjects, or between subgroups of patients created on the basis of clinical characteristics. In logistic regression analysis, the high number of recurrences (>10) of AAU was independently related to the presence of single or multiple bacterial antibodies (p=0.04). None of the PBMC samples of the patients were positive for Yersinia or Salmonella antigens. C pneumoniae PCR was positive in a patient who was negative for C pneumoniae antibodies.
Although neither the prevalence nor the levels of single microbial antibodies studied differed between the patients and the controls, current data suggest that the presence of single or multiple antibodies in patients with many recurrences of AAU compared with patients with none or few recurrences may be a sign of repeated infections, antigen persistence, or raised innate immune responsiveness.
研究急性前葡萄膜炎（AAU）病史患者及健康对照者的感染背景。
对64例既往有AAU病史的患者和64例年龄及性别匹配的对照者进行研究。采用酶联免疫吸附测定（ELISA）检测血清中针对沙门氏菌、耶尔森氏菌、肺炎克雷伯菌、大肠杆菌、奇异变形杆菌、空肠弯曲菌和伯氏疏螺旋体的抗体，通过微量免疫荧光试验检测沙眼衣原体和肺炎衣原体的抗体。通过密度梯度离心分离外周血单个核细胞（PBMC），采用免疫荧光试验研究其中沙门氏菌和耶尔森氏菌抗原，采用聚合酶链反应（PCR）检测肺炎衣原体DNA。
所研究的单一微生物抗体的患病率和水平在患者与对照者之间，或根据临床特征划分的患者亚组之间均无差异。在逻辑回归分析中，AAU复发次数较多（>10次）与存在单一或多种细菌抗体独立相关（p=0.04）。患者的PBMC样本中耶尔森氏菌或沙门氏菌抗原均为阴性。1例肺炎衣原体抗体阴性的患者肺炎衣原体PCR检测呈阳性。
虽然所研究的单一微生物抗体的患病率和水平在患者与对照者之间无差异，但目前的数据表明，与无复发或复发次数少的患者相比，AAU复发次数多的患者存在单一或多种抗体可能是反复感染、抗原持续存在或先天性免疫反应性升高的迹象。
