Assem M, Teyssier J R, Benderitter M, Terrand J, Laubriet A, Javouhey A, David M, Rochette L
Laboratory of Cardiovascular Physiopathology and Pharmacology, University Hospital, Dijon, France.
Am J Pathol. 1997 Aug;151(2):549-55.
A multiplex reverse transcription polymerase chain reaction assay was designed to measure manganese superoxide dismutase (MnSOD) and CuZnSOD mRNAs in the left and right ventricles of rat hearts after myocardial infarction induced by occlusion of the left coronary artery. These data were compared with changes in enzymatic activities. In the left ventricle, Mn-SOD RNA increased significantly at 6 hours, peaked at 12 hours (490 +/- 38 arbitrary units), and progressively decreased (127 +/- 21 arbitrary units at 48 hours). In contrast, there was a steady accumulation of transcripts in the right ventricle up to 48 hours. In both ventricles, the changes in the MnSOD mRNA and protein content were not associated with proportional variations in enzymatic activity. There was no characteristic alteration of the CuZnSOD system in either ventricle over the 48-hour period. These results demonstrate that infarction selectively activates the MnSOD gene in the viable myocardium of both ventricles. They suggest that MnSOD may be involved in the adaptive response of myocytes to the overloading stress.
设计了一种多重逆转录聚合酶链反应检测方法，以测定左冠状动脉闭塞诱导心肌梗死后大鼠心脏左、右心室中锰超氧化物歧化酶（MnSOD）和铜锌超氧化物歧化酶（CuZnSOD）的mRNA。将这些数据与酶活性的变化进行比较。在左心室，Mn-SOD RNA在6小时时显著增加，在12小时时达到峰值（490±38任意单位），并逐渐下降（48小时时为127±21任意单位）。相比之下，右心室中的转录本在48小时内持续积累。在两个心室中，MnSOD mRNA和蛋白质含量的变化与酶活性的比例变化无关。在48小时内，两个心室中的CuZnSOD系统均无特征性改变。这些结果表明，梗死选择性地激活了两个心室存活心肌中的MnSOD基因。它们提示MnSOD可能参与了心肌细胞对过载应激的适应性反应。
