大肠杆菌中钴胺素依赖性甲硫氨酸合酶的纯化与测定 - Suppr

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超能文献

Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli.

作者信息

Jarrett J T, Goulding C W, Fluhr K, Huang S, Matthews R G

机构信息

Biophysics Research Division, University of Michigan, Ann Arbor 48109-1055, USA.

出版信息

Methods Enzymol. 1997;281:196-213. doi: 10.1016/s0076-6879(97)81026-9.

DOI:10.1016/s0076-6879(97)81026-9

PMID:9250984

Abstract

摘要

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Purification and assay of cobalamin-dependent methionine synthase from Escherichia coli.大肠杆菌中钴胺素依赖性甲硫氨酸合酶的纯化与测定

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Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin, and adenosylmethionine.钴胺素依赖性甲硫氨酸合酶是一种模块化蛋白质，具有用于结合同型半胱氨酸、甲基四氢叶酸、钴胺素和腺苷甲硫氨酸的不同区域。

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Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem.来自大肠杆菌的钴胺素非依赖性和钴胺素依赖性甲硫氨酸合酶的比较：针对同一化学问题的两种解决方案。

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Assignment of enzymatic function to specific protein regions of cobalamin-dependent methionine synthase from Escherichia coli.将酶功能赋予来自大肠杆菌的钴胺素依赖性甲硫氨酸合酶的特定蛋白质区域。

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