嗜热栖热放线菌木聚糖酶在乳酸克鲁维酵母中的表达与分泌
Expression and secretion of a thermostable bacterial xylanase in Kluyveromyces lactis.
作者信息
Walsh D J, Bergquist P L
机构信息
Centre for Gene Technology, University of Auckland, New Zealand.
出版信息
Appl Environ Microbiol. 1997 Aug;63(8):3297-300. doi: 10.1128/aem.63.8.3297-3300.1997.
Abstract
The xynA structural gene from the extremely thermophilic anaerobe Dictyoglomus thermophilum Rt46B.1 was fused in frame with the secretion signal of the Kluyveromyces lactis killer toxin in episomal expression vectors based on the Kluyveromyces plasmid pKD1. XynA was secreted predominantly as an unglycosylated 35-kDa protein which comprised up to 90% of the total extracellular proteins and reached a concentration of 130 micrograms/ml in shake-flask cultures grown under selective conditions.
摘要
将嗜热厌氧菌嗜热栖热袍菌（Dictyoglomus thermophilum）Rt46B.1的木聚糖酶A（xynA）结构基因与乳酸克鲁维酵母（Kluyveromyces lactis）杀伤毒素的分泌信号框内融合，构建于基于克鲁维酵母质粒pKD1的附加型表达载体中。木聚糖酶A主要以未糖基化的35 kDa蛋白形式分泌，其占细胞外总蛋白的比例高达90%，在选择性条件下摇瓶培养时浓度可达130微克/毫升。
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