蛋白胨和酪氨酸对木质素过氧化物酶测定的干扰。
Interference of peptone and tyrosine with the lignin peroxidase assay.
作者信息
ten Have R, Hartmans S, Field J A
机构信息
Department of Food Science, Wageningen Agricultural University, The Netherlands.
出版信息
Appl Environ Microbiol. 1997 Aug;63(8):3301-3. doi: 10.1128/aem.63.8.3301-3303.1997.
Abstract
The N-unregulated white rot fungus Bjerkandera sp. strain BOS55 was cultured in 1 liter of peptone-yeast extract medium to produce lignin peroxidase (LiP). During the LiP assay, the oxidation of veratryl alcohol to veratraldehyde was inhibited due to tyrosine present in the peptone and the yeast extract.
摘要
未调控的白腐真菌Bjerkandera sp.菌株BOS55在1升蛋白胨 - 酵母提取物培养基中培养以产生木质素过氧化物酶（LiP）。在LiP测定过程中，由于蛋白胨和酵母提取物中存在酪氨酸，藜芦醇向藜芦醛的氧化受到抑制。
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