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四氢可的松-3-β-D-葡萄糖醛酸苷在尿17-羟皮质类固醇测定中的应用。

The use of tetrahydrocortisone-3-beta-D-glucuronide in the measurement of urinary 17-hydroxycorticosteroids.

作者信息

Hadd H E

出版信息

J Clin Endocrinol Metab. 1977 Nov;45(5):1128-30. doi: 10.1210/jcem-45-5-1128.

DOI:10.1210/jcem-45-5-1128
PMID:925139
Abstract

THE 3-G was added to H2O and 56 urines, and the recovered THE was measured by the Porter-Silber method. The recovery from H2O was quantitative (98 +/- 2%), but highly variables from urine, ranging from 35 to 100%. The necessity of the proper standard in analysis of urinary steroid glucuronides was demonstrated. The presence in urine of endogenous inhibitors to beta-glucuronidase was confirmed.

摘要

将3-G加入到水和56份尿液中,然后用波特-西尔伯法测定回收的3-G。从水中的回收率是定量的(98±2%),但从尿液中的回收率变化很大,范围为35%至100%。这证明了在分析尿甾体葡萄糖醛酸苷时使用适当标准的必要性。证实了尿液中存在β-葡萄糖醛酸酶的内源性抑制剂。

相似文献

1
The use of tetrahydrocortisone-3-beta-D-glucuronide in the measurement of urinary 17-hydroxycorticosteroids.四氢可的松-3-β-D-葡萄糖醛酸苷在尿17-羟皮质类固醇测定中的应用。
J Clin Endocrinol Metab. 1977 Nov;45(5):1128-30. doi: 10.1210/jcem-45-5-1128.
2
[Determination method for urinary 17-OHCS (Porter-Silber chromogen). 1. Effect of sodium sulfate on beta-glucuronidase catalyzed hydrolysis].[尿17-羟皮质类固醇(波特-西尔伯色原)的测定方法。1. 硫酸钠对β-葡萄糖醛酸酶催化水解的影响]
Rinsho Byori. 1969 Aug;17(8):634-8.
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Effect of sodium sulfate on the hydrolysis of 17-hydroxycorticosteroid- and p-nitrophenyl-glucuronides with beta-glucuronidase preparations from bovine liver.硫酸钠对用牛肝β-葡萄糖醛酸酶制剂水解17-羟基皮质类固醇和对硝基苯基葡萄糖醛酸苷的影响。
Clin Chem. 1977 Jun;23(6):982-5.
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The effect of sodium bisulfite on the removal of drugs and their metabolites interfering with the Porter-Silber reaction in the determination of urinary 17-hydroxycorticosteroids.亚硫酸氢钠对在尿17-羟皮质类固醇测定中干扰波特-西尔伯反应的药物及其代谢物去除的影响。
Am J Clin Pathol. 1975 Sep;64(3):315-26. doi: 10.1093/ajcp/64.3.315.
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Improved hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase from Helix pomatia, on adding sodium sulfate.添加硫酸钠后,用来自苹果螺的β-葡萄糖醛酸酶可提高尿中17-羟基皮质类固醇葡萄糖醛酸苷的水解率。
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A fluorometric determination of urinary 17-hydroxycorticosteroids using benzamidine.使用苯甲脒荧光法测定尿中17-羟皮质类固醇
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Hydrolysis of steroid glucuronides with beta-glucuronidase preparations from bovine liver, Helix pomatia, and E. coli.用来自牛肝、苹果螺和大肠杆菌的β-葡萄糖醛酸酶制剂水解甾体葡萄糖醛酸苷。
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Ann Clin Biochem. 2001 May;38(Pt 3):277-9. doi: 10.1258/0004563011900524.
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[Continuous flow system for the determination of urinary 17-hydroxycorticosteroids (Porter-Silber chromogens) by autoanalyzer].[用自动分析仪测定尿中17-羟皮质类固醇(波特-西尔伯色原)的连续流动系统]
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17-Hydroxycorticosteroids in urine.尿液中的17-羟皮质类固醇
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