四氢可的松-3-β-D-葡萄糖醛酸苷在尿17-羟皮质类固醇测定中的应用。
The use of tetrahydrocortisone-3-beta-D-glucuronide in the measurement of urinary 17-hydroxycorticosteroids.
作者信息
Hadd H E
出版信息
J Clin Endocrinol Metab. 1977 Nov;45(5):1128-30. doi: 10.1210/jcem-45-5-1128.
PMID:925139
Abstract
THE 3-G was added to H2O and 56 urines, and the recovered THE was measured by the Porter-Silber method. The recovery from H2O was quantitative (98 +/- 2%), but highly variables from urine, ranging from 35 to 100%. The necessity of the proper standard in analysis of urinary steroid glucuronides was demonstrated. The presence in urine of endogenous inhibitors to beta-glucuronidase was confirmed.
摘要
将3-G加入到水和56份尿液中，然后用波特-西尔伯法测定回收的3-G。从水中的回收率是定量的（98±2%），但从尿液中的回收率变化很大，范围为35%至100%。这证明了在分析尿甾体葡萄糖醛酸苷时使用适当标准的必要性。证实了尿液中存在β-葡萄糖醛酸酶的内源性抑制剂。
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