Simon M, Babich-Armstrong M
Clin Chim Acta. 1980 Apr 11;103(1):101-8. doi: 10.1016/0009-8981(80)90235-1.
A new method has been devised to extract 17-hydroxycorticosteroids (17OH-CS) from a hydrolyzed urine using an EXTUBE fiber column. This extraction was combined with a modified Porter-Silber color reaction to quantitate total urinary 17OH-CS. The new method takes only half the time required in older methods. Recovery of tetrahydrocortisone in the new method was improved to 85--95% compared with 70% in the previous method. Between-run precision (C.V.%) was concentration dependent in both methods and was improved to 5--7% compared with 10--18% in the previous method. Evidence was obtained to demonstrate that the new methodology would provide the same information and interpretation as the older, more cumbersome method.
已设计出一种新方法，使用EXTUBE纤维柱从水解尿液中提取17-羟基皮质类固醇（17OH-CS）。该提取方法与改良的波特-西尔伯显色反应相结合，用于定量测定尿中总17OH-CS。新方法所需时间仅为旧方法的一半。新方法中四氢可的松的回收率提高到了85%-95%，而之前的方法为70%。两种方法的批间精密度（变异系数%）均与浓度有关，新方法的批间精密度提高到了5%-7%，而之前的方法为10%-18%。有证据表明，新方法能提供与旧的、更繁琐的方法相同的信息和解释。
