Zhang J F, Zheng F
Department of Physiology, Xuzhou Medical College, Jiangsu, Peoples Republic of China.
Brain Res. 1997 Jul 4;761(2):203-9. doi: 10.1016/s0006-8993(97)00257-6.
The rat stress model of restraint and cold water immersion was used to investigate the effect of stimulating the paraventricular nucleus (PVN) of hypothalamus on the development of stress-induced gastric ulceration. The results were (1) electric stimulation of the PVN increased the stress ulceration, while electrolytic lesion of the PVN decreased it; (2) intracerebroventricular injection (i.c.v.) of acetylcholine (Ach) enhanced the effect of PVN stimulation on stress ulcers, and the M-receptor was involved; (3) i.c.v. norepinephrine (NE) attenuated the effect of PVN stimulation on stress ulcers in a dose-dependent manner, and the beta-receptor was involved; (4) i.c.v. 5-hydroxytryptamine (5-HT) enhanced the effect of PVN stimulation on stress ulcers; (5) electrolytic lesions of dorsal raphe nucleus (DR) attenuated the effect of PVN stimulation on stress ulcers, while electrolytic lesions of the locus ceruleus (LC) aggravated the effect; (6) thyroidectomy, adrenalectomy, ovariectomy, vagotomy and sympathectomy all attenuated the effect of PVN stimulation on stress ulcers; (7) electric stimulation of the PVN produced no effect on gastric juice volume, acidity, total acid output, pepsin activity or the gastric barrier mucus; but greatly reduced gastric mucosal blood flow. These results indicate that the PVN is an important brain site regulating the development of stress-induced gastric ulcers, that the classical neurotransmitters Ach, NE and 5-HT are involved, and that in the periphery, both the parasympathetic and sympathetic nervous systems and the three endocrine glands (thyroid, adrenal and gonad) take part in the effect.
采用束缚加冷水浸泡的大鼠应激模型，研究刺激下丘脑室旁核（PVN）对应激性胃溃疡形成的影响。结果如下：（1）电刺激PVN会增加应激性溃疡，而PVN的电解损伤则会使其减少；（2）脑室内注射（i.c.v.）乙酰胆碱（Ach）增强了PVN刺激对应激性溃疡的作用，且涉及M受体；（3）脑室内注射去甲肾上腺素（NE）以剂量依赖方式减弱了PVN刺激对应激性溃疡的作用，且涉及β受体；（4）脑室内注射5-羟色胺（5-HT）增强了PVN刺激对应激性溃疡的作用；（5）中缝背核（DR）的电解损伤减弱了PVN刺激对应激性溃疡的作用，而蓝斑（LC）的电解损伤则加剧了该作用；（6）甲状腺切除术、肾上腺切除术、卵巢切除术、迷走神经切断术和交感神经切除术均减弱了PVN刺激对应激性溃疡的作用；（7）电刺激PVN对胃液量、酸度、总酸排出量、胃蛋白酶活性或胃屏障黏液没有影响，但大大减少了胃黏膜血流量。这些结果表明，PVN是调节应激性胃溃疡形成的重要脑区，经典神经递质Ach、NE和5-HT参与其中，且在外周，副交感神经系统和交感神经系统以及三个内分泌腺（甲状腺、肾上腺和性腺）均参与了该效应。
