Patel Nikunj B, Galani Varsha J, Patel Bharatkumar G
Department of Pharmacology, A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar, Gujarat, India.
J Ayurveda Integr Med. 2011 Jul;2(3):129-36. doi: 10.4103/0975-9476.85551.
The antistress effect of a seven-day treatment (100 and 200 mg / kg, p.o.) of the hydroalcoholic extract of Argyreia speciosa root (ASE) was evaluated by using the swimming endurance test, acetic acid-induced writhing test, pentylenetetrazole-induced convulsion test, anoxic tolerance test, cold-restraint, stress-induced gastric ulcers, aspirin-induced ulcers, and biochemical, and histopathological changes in the cold-restraint stress test. The immunomodulatory activity was also evaluated for the same doses, and treatment of ASE was done using the hemagglutination test. Both the doses of ASE showed antistress activity in all the tested models. The ASE-treated animals showed a decrease in immobility time and an increase in anoxic tolerance time in swimming endurance and the anoxic tolerance tests, respectively. The effect of glacial acetic acid and pentylenetetrazole were also reduced by decreasing the number of writhing responses and increasing the onset of convulsions, respectively. In the cold restrained stress and aspirin-induced gastric ulcer models, ASE showed a significant reduction in the ulcer index. Pretreatment with ASE significantly ameliorated the cold stress-induced variations in biochemical levels such as increased plasma cholesterol, triglyceride, glucose, total protein, and cortisol. ASE was also effective in preventing the pathological changes in the adrenal gland, due to cold restrained stress, in rats. In mice immunized with sheep red blood cells, the treatment groups subjected to restraint stress prevented the humoral immune response to the antigen. The immunostimulating activity of the ASE was indicated by an increase in the antibody titer in mice pre-immunized with sheep red blood cells and subjected to restraint stress. The findings of the present investigations indicate that the ASE has significant antistress activity, which may be due to the immunostimulating property and increased resistance, nonspecifically, against all experimental stress conditions.
通过游泳耐力试验、醋酸诱导扭体试验、戊四氮诱导惊厥试验、耐缺氧试验、冷束缚应激诱导胃溃疡试验、阿司匹林诱导胃溃疡试验以及冷束缚应激试验中的生化和组织病理学变化，评估了银背叶旋花根水醇提取物（ASE）连续7天治疗（100和200 mg/kg，口服）的抗应激作用。还对相同剂量的ASE进行了免疫调节活性评估，并使用血凝试验进行了ASE治疗。ASE的两种剂量在所有测试模型中均显示出抗应激活性。在游泳耐力试验和耐缺氧试验中，经ASE处理的动物分别表现出不动时间减少和耐缺氧时间增加。通过分别减少扭体反应次数和增加惊厥发作时间，也降低了冰醋酸和戊四氮的作用。在冷束缚应激和阿司匹林诱导的胃溃疡模型中，ASE显示溃疡指数显著降低。ASE预处理显著改善了冷应激引起的生化水平变化，如血浆胆固醇、甘油三酯、葡萄糖、总蛋白和皮质醇升高。ASE还能有效预防大鼠因冷束缚应激引起的肾上腺病理变化。在绵羊红细胞免疫的小鼠中，遭受束缚应激的治疗组抑制了对抗原的体液免疫反应。在预先用绵羊红细胞免疫并遭受束缚应激的小鼠中，抗体滴度增加表明了ASE的免疫刺激活性。本研究结果表明，ASE具有显著的抗应激活性，这可能归因于其免疫刺激特性以及非特异性地增强了对所有实验应激条件的抵抗力。
