Viazov S, Riffelmann M, Sarr S, Ballauff A, Meisel H, Roggendorf M
Institute of Virology, Universityklinikum Essen, Germany.
J Hepatol. 1997 Jul;27(1):85-90. doi: 10.1016/s0168-8278(97)80284-x.
BACKGROUND/AIMS: In 1995, a new flavivirus, GBV-C/HGV was identified. Little information is available on the clinical manifestations and epidemiology of GBV-C/HGV infection. We investigated the risk of mother-to-infant transmission in a group of GBV-C/HGV RNA positive women and followed up the GBV-C/HGV infected babies.
Twenty-eight anti-HCV positive women, of whom 25 have been intravenous drug users, and their children were included in the study. RNA was extracted from serum, reverse transcribed and amplified with primers from the NS5 region of GBV-C/HGV and 5'-UTR of HCV in a nested polymerase chain reaction. Amplified DNA fragments were gel purified and sequenced; the sequences obtained were subjected to a phylogenetic analysis.
Transmission of GBV-C/HGV occurred in 10 (56%) of 18 infants born to GBV-C/HGV positive mothers; all these women were drug abusers. Only one (5%) of 19 babies whose mothers were HCV RNA positive by polymerase chain reaction, was infected with HCV during the follow up. High sequence homology in the NS5 region of GBV-C/HGV isolates in 10 mother-child pairs suggested mother-to-infant transmission. All 10 babies remained GBV-C/HGV RNA positive during follow up (2-12 months). None of the GBV-C/HGV infected infants became icteric or demonstrated any clinical or biochemical signs of liver disease.
Mother-to-infant transmission of GBV-C/HGV may be high, at least in HCV-infected, drug-addicted women. In GBV-C/HGV RNA positive infants the rate of GBV-C/HGV persistent infection is high, but the infection is not accompanied by any symptoms of liver disease.
背景/目的：1995年，一种新的黄病毒——GBV-C/HGV被发现。关于GBV-C/HGV感染的临床表现和流行病学的信息很少。我们调查了一组GBV-C/HGV RNA阳性女性的母婴传播风险，并对感染GBV-C/HGV的婴儿进行了随访。
28名抗-HCV阳性女性及其子女被纳入研究，其中25名曾是静脉吸毒者。从血清中提取RNA，在巢式聚合酶链反应中用GBV-C/HGV NS5区域的引物和HCV的5'-UTR进行逆转录和扩增。扩增的DNA片段经凝胶纯化和测序；对获得的序列进行系统发育分析。
18名GBV-C/HGV阳性母亲所生的婴儿中有10名（56%）发生了GBV-C/HGV传播；所有这些女性都是吸毒者。在随访期间，19名母亲通过聚合酶链反应检测HCV RNA呈阳性的婴儿中只有1名（5%）感染了HCV。10对母婴中GBV-C/HGV分离株NS5区域的高序列同源性提示母婴传播。所有10名婴儿在随访期间（2至12个月）GBV-C/HGV RNA仍为阳性。没有一名感染GBV-C/HGV的婴儿出现黄疸或表现出任何肝病的临床或生化体征。
GBV-C/HGV的母婴传播可能很高，至少在感染HCV的吸毒女性中如此。在GBV-C/HGV RNA阳性婴儿中，GBV-C/HGV持续感染率很高，但感染并未伴有任何肝病症状。
