Seemayer C A, Viazov S, Philipp T, Roggendorf M
Institut für Virologie, Universitätsklinikum Essen, Germany.
Infection. 1998 Jan-Feb;26(1):39-41. doi: 10.1007/BF02768751.
Saliva and urine samples from six GB virus C (GBV-C)/hepatitis G virus (HGV)-infected renal transplant patients were tested by RT-PCR. Viral RNA was detected in all saliva samples, but the viral RNA titers in saliva were 100 to 10,000 lower than those in the corresponding sera. Comparative sequence analysis of the amplified 354 bp DNA from one patient revealed full identity of GBV-C/HGV variants present in serum and saliva. None of the urine samples from the six patients was found to contain GBV-C/HGV RNA. High prevalence of GBV-C/HGV RNA in saliva of infected individuals may contribute to a wide spread of GBV-C/HGV infection, at least in some settings.
采用逆转录聚合酶链反应（RT-PCR）对6例感染GB病毒C型（GBV-C）/庚型肝炎病毒（HGV）的肾移植患者的唾液和尿液样本进行检测。在所有唾液样本中均检测到病毒RNA，但唾液中的病毒RNA滴度比相应血清中的低100至10000倍。对1例患者扩增出的354 bp DNA进行比较序列分析，结果显示血清和唾液中存在的GBV-C/HGV变异体完全相同。6例患者的尿液样本均未检测到GBV-C/HGV RNA。感染个体唾液中GBV-C/HGV RNA的高流行率可能至少在某些情况下导致GBV-C/HGV感染的广泛传播。
