Chary V K, Amaya E I, Piggot P J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140, USA.
FEMS Microbiol Lett. 1997 Aug 1;153(1):135-9. doi: 10.1111/j.1574-6968.1997.tb10474.x.
A plasmid is described for Bacillus subtilis that facilitates replacement of the widely used neomycin resistance gene (neo) with a spectinomycin resistance (spcE) gene. A second plasmid is described that facilitates replacement of spcS, associated with mini-Tn10 mutagenesis in B. subtilis, with neo. These plasmids can also function as integrative vectors for B. subtilis. They expand the scope of strain construction and gene analysis in B. subtilis.
描述了一种用于枯草芽孢杆菌的质粒，该质粒有助于用壮观霉素抗性（spcE）基因替换广泛使用的新霉素抗性基因（neo）。还描述了第二种质粒，它有助于用neo替换与枯草芽孢杆菌中的mini-Tn10诱变相关的spcS。这些质粒也可以作为枯草芽孢杆菌的整合载体发挥作用。它们扩大了枯草芽孢杆菌中菌株构建和基因分析的范围。
