Fernandes-Carlos T, Riondel J, Glise D, Guiraud P, Favier A
Groupe de Recherche et d'Etude des Pathologies Oxydatives, Faculté de Pharmacie, La Tronche, France.
Anticancer Res. 1997 Jul-Aug;17(4A):2523-7.
In athymic mice, Natural Killer (NK) cells influence the take rate and growth of human malignant tissue xenografts. To confirm preliminary results, comparative experiments were conducted to study the effects of beta-carotene, oestrone and their association on the cytolysis mediated by spleen NK cells from athymic mice receiving these different treatments. Target cells consisted of YAC-1 malignant cells. With a 65% increase of cytolysis (ratio effector/target 50:1), beta-carotene induced a significant activation of NK cells (p < 0.002). This effect could be attributed to its antioxidant properties and confirmed by a moderate increase in erythrocyte glutathione peroxidase activity. On the contrary, oestrone resulted in a significant decrease of cytolysis (p < 0.001). In this case, the prooxidant properties of oestrone could explain its effect on NK cells and agree with the increase of intracellular reduced glutathione level observed. When mice received the combination beta-carotene-oestrone, their opposite effects on NK cell activity were counterbalanced, leading to a moderate change of cytolysis.
在无胸腺小鼠中，自然杀伤（NK）细胞会影响人类恶性组织异种移植的植入率和生长情况。为了证实初步结果，进行了对比实验，以研究β-胡萝卜素、雌酮及其组合对接受这些不同处理的无胸腺小鼠脾脏NK细胞介导的细胞溶解作用的影响。靶细胞由YAC-1恶性细胞组成。β-胡萝卜素使细胞溶解增加了65%（效应细胞/靶细胞比例为50:1），诱导NK细胞显著激活（p < 0.002）。这种效应可能归因于其抗氧化特性，红细胞谷胱甘肽过氧化物酶活性适度增加也证实了这一点。相反，雌酮导致细胞溶解显著降低（p < 0.001）。在这种情况下，雌酮的促氧化特性可以解释其对NK细胞的作用，并且与观察到的细胞内还原型谷胱甘肽水平升高一致。当小鼠接受β-胡萝卜素 - 雌酮组合时，它们对NK细胞活性的相反作用相互抵消，导致细胞溶解有适度变化。
