Carlos T F, Riondel J, Mathieu J, Guiraud P, Mestries J C, Favier A
Groupe de Recherches et d'Etudes des Pathologies Oxydatives (GREPO), Faculté de Pharmacie, La Tronche, France.
In Vivo. 1997 Jan-Feb;11(1):87-91.
To study the effects of beta-carotene on Natural Killer (NK) cells, we chose athymic mice whose spleens have a higher percentage of NK cells than conventional mice. Preliminary studies conducted with beta-carotene given intraperitoneally to athymic mice xenografted with a small-cell lung carcinoma resulted in a slight but significant antiproliferative effect (unpublished observations). We speculated that such an activity of beta-carotene was related to its immunostimulating properties. NK cell activity in ungrafted athymic mice as influenced by beta-carotene was studied. Mice received beta-carotene intraperitoneally. Splenic NK cells were labelled with monoclonal antibody and numeration was completed by measurement of their functional activity against YAC-1 malignant cells with a 51Cr release assay. In addition, splenic lymphocytes were evaluated for their reduced glutathione (GSH) content. There was a non-significant increase in the number of NK cells in the spleen, however their killing capacity was significantly (p < 0.01) enhanced after beta-carotene treatment. Also the GSH content of splenic lymphocytes was significantly higher in beta-carotene treated mice. Comparison of the average body weights of treated animals and of their respective controls showed that treatment had no adverse effects.
为了研究β-胡萝卜素对自然杀伤（NK）细胞的影响，我们选择了无胸腺小鼠，其脾脏中NK细胞的百分比高于传统小鼠。对移植了小细胞肺癌的无胸腺小鼠腹腔注射β-胡萝卜素进行的初步研究产生了轻微但显著的抗增殖作用（未发表的观察结果）。我们推测β-胡萝卜素的这种活性与其免疫刺激特性有关。研究了β-胡萝卜素对未移植无胸腺小鼠NK细胞活性的影响。小鼠腹腔注射β-胡萝卜素。用单克隆抗体标记脾脏NK细胞，并通过用51Cr释放试验测量其对YAC-1恶性细胞的功能活性来完成计数。此外，评估脾脏淋巴细胞的谷胱甘肽（GSH）含量。脾脏中NK细胞的数量有不显著的增加，然而，β-胡萝卜素处理后它们的杀伤能力显著增强（p < 0.01）。β-胡萝卜素处理的小鼠脾脏淋巴细胞的GSH含量也显著更高。比较处理动物与其各自对照的平均体重表明，处理没有不良影响。
