Hornícková Z
National Institute of Public Health, Department of Epidemiology and Microbiology, Czech Republic.
Cell Biochem Funct. 1997 Jun;15(2):87-93. doi: 10.1002/(SICI)1099-0844(19970601)15:2<87::AID-CBF726>3.0.CO;2-6.
The effect of influenza strains A (H3N2) and B, isolated during the seasons of 1994 and 1995 in the Czech Republic, on MDCK cells was studied. Various concentrations of virus and conditions of nutrition were used during the cell culture. The virus replication and consequently fragmentation of genomic DNA together with cytotoxicity were investigated in the absence and presence of 10 per cent calf serum. Virus replication, regardless of type A or B, caused earlier DNA fragmentation in comparison to non-infected cells in tissue culture. The results showed that the influenza B strain had a greater cytotoxic effect on MDCK cells than influenza A. A higher infection dose of influenza A virus accelerated the onset of apoptosis; conversely, a higher infection dose of influenza B virus delayed the onset of apoptosis. The absence of serum enhanced the progress of influenza-induced apoptosis in conditions in vitro.
研究了1994年和1995年在捷克共和国分离出的甲型（H3N2）和乙型流感毒株对MDCK细胞的影响。细胞培养过程中使用了不同浓度的病毒和营养条件。在有和没有10%小牛血清的情况下，研究了病毒复制以及由此导致的基因组DNA片段化和细胞毒性。与组织培养中未感染的细胞相比，无论甲型还是乙型病毒，病毒复制都会导致更早的DNA片段化。结果表明，乙型流感毒株对MDCK细胞的细胞毒性作用比甲型流感毒株更大。更高感染剂量的甲型流感病毒加速了细胞凋亡的发生；相反，更高感染剂量的乙型流感病毒延迟了细胞凋亡的发生。在体外条件下，血清的缺乏增强了流感诱导的细胞凋亡进程。
