Rosa L F
Department of Histology and Embryology, Biomedical Sciences Institute, University of São Paulo, Brasil.
Cell Biochem Funct. 1997 Jun;15(2):103-12.
This study examined the effect of adrenaline on lymphocyte metabolism and function. The following parameters were addressed: cell proliferation, glucose and glutamine metabolism as indicated by the measurement of enzyme activities, the utilization of metabolites and production and oxidation of substrates. We also evaluated the involvement of beta-receptors in this response as well as the possible effect of cAMP and hydrogen peroxide in the process of lymphocyte activation by adrenaline. The results indicated that adrenaline is able to induce metabolic changes in lymphocytes that are related to enhanced proliferative capacity, but under physiological conditions fails to initiate the process, the catecholamine could, increase cell proliferation via increased production of H2O2 by macrophages, since this reactive oxygen intermediate can act as a trigger for lymphocyte activation. The results also showed that distinct populations of lymphocytes present different responses to adrenaline activation, as demonstrated by cells obtained from the same site but exposed to different mitogens such as LPS and ConA.
本研究考察了肾上腺素对淋巴细胞代谢和功能的影响。研究涉及以下参数：细胞增殖、通过酶活性测定所表明的葡萄糖和谷氨酰胺代谢、代谢物的利用以及底物的产生和氧化。我们还评估了β受体在这一反应中的参与情况，以及环磷酸腺苷（cAMP）和过氧化氢在肾上腺素激活淋巴细胞过程中的可能作用。结果表明，肾上腺素能够诱导淋巴细胞发生与增殖能力增强相关的代谢变化，但在生理条件下无法启动这一过程，儿茶酚胺可通过巨噬细胞增加过氧化氢的产生来促进细胞增殖，因为这种活性氧中间体可作为淋巴细胞激活的触发因素。结果还表明，不同群体的淋巴细胞对肾上腺素激活呈现出不同的反应，从同一部位获取但暴露于不同促有丝分裂原（如脂多糖和刀豆蛋白A）的细胞就证明了这一点。
