Rosa L F, Safi D A, Curi R
Department of Physiology and Biophysics, University of São Paulo, Brazil.
Cell Biochem Funct. 1995 Jun;13(2):141-7. doi: 10.1002/cbf.290130211.
The effect of thyroid hormones on monocyte migration, phagocytic capacity and hydrogen peroxide production by macrophages and the effect of these hormones on glutamine and glucose metabolism was investigated. The experiments were performed on resident, thioglycollate- and BCG-stimulated cells from hypo- and hyperthyroid rats. High plasma levels of thyroid hormones suppressed the migration of monocytes and hydrogen peroxide production, whereas hypothyroidism did not affect cell migration but raised the phagocytic capacity and the hydrogen peroxide production. Hyperthyroidism increased the activities of glutaminase and hexokinase and the rates of decarboxylation of [U-14C]-glutamine and [U-14C]-glucose in inflammatory and activated cells. Hypothyroidism stimulated glucose metabolism and had only a slight effect on glutaminolysis. The activity of the TCA cycle was, however, diminished in the presence of high plasma levels of thyroid hormones and enhanced by the hypothyroid state. These findings suggest that the functional changes are more likely to be related to the activity of the TCA cycle rather than to glutaminolysis and glycolysis.
研究了甲状腺激素对单核细胞迁移、巨噬细胞吞噬能力和过氧化氢生成的影响，以及这些激素对谷氨酰胺和葡萄糖代谢的影响。实验在甲状腺功能减退和亢进大鼠的驻留细胞、经巯基乙酸盐和卡介苗刺激的细胞上进行。高血浆水平的甲状腺激素抑制单核细胞迁移和过氧化氢生成，而甲状腺功能减退不影响细胞迁移，但提高了吞噬能力和过氧化氢生成。甲状腺功能亢进增加了炎症细胞和活化细胞中谷氨酰胺酶和己糖激酶的活性以及[U-14C]-谷氨酰胺和[U-14C]-葡萄糖的脱羧速率。甲状腺功能减退刺激葡萄糖代谢，对谷氨酰胺分解仅有轻微影响。然而，在高血浆甲状腺激素水平存在时，三羧酸循环的活性降低，而甲状腺功能减退状态则增强了该活性。这些发现表明，功能变化更可能与三羧酸循环的活性有关，而不是与谷氨酰胺分解和糖酵解有关。
