Fukada H, Hiramatsu N, Gen K, Hara A
Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1997 Jul;117(3):387-92. doi: 10.1016/s0305-0491(97)00134-x.
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of low levels of serum growth hormone (GH) in chum salmon (Oncorhynchus keta). The antiserum to GH (a-rsGH) was obtained from a rabbit immunized with recombinant chum salmon GH. The noncompetitive ELISA was performed by a sandwich method using a-rsGH rabbit IgG as the first antibody, its biotinylated Fab' fragment as the second antibody, and the avidin-biotin reaction for signal amplification. This assay could be run in 3 days and routinely detected GH at concentrations as low as 0.5 ng/ml. The development of an ELISA for GH made possible quantification of serum GH levels. In this assay system, parallel dilution curves were obtained using purified chum salmon GH and GH's from several species of the genus Oncorhynchus.
开发了一种特异性和灵敏的酶联免疫吸附测定法（ELISA），用于测量大麻哈鱼（Oncorhynchus keta）血清中低水平的生长激素（GH）。抗GH血清（a-rsGH）取自用重组大麻哈鱼GH免疫的兔子。非竞争性ELISA采用夹心法进行，以a-rsGH兔IgG作为第一抗体，其生物素化的Fab'片段作为第二抗体，并利用抗生物素蛋白-生物素反应进行信号放大。该测定法可在3天内完成，常规检测低至0.5 ng/ml浓度的GH。用于GH的ELISA的开发使得血清GH水平的定量成为可能。在该测定系统中，使用纯化的大麻哈鱼GH和几种大麻哈鱼属物种的GH获得了平行稀释曲线。
