Castelli F, Trombetta D, Tomaino A, Bonina F, Romeo G, Uccella N, Saija A
Department of Chemical Sciences, University of Catania, Italy.
J Pharmacol Toxicol Methods. 1997 Apr;37(3):135-41. doi: 10.1016/s1056-8719(97)00009-9.
Large unilamellar vesicles (LUVs) are generally accepted to be a suitable model for peroxidation studies. In the present report, dipalmitoylphosphatidylcholine (DPPC)/linoleic acid-mixed LUVs were employed as model membranes to verify the inhibitory effect of tocopherol (an efficient representative antioxidant) against 2,2'-azobis(2-amidinopropane)hydrochloride-induced peroxidation (evaluated by monitoring conjugated diene accumulation). In this model, the appropriate experimental conditions (particularly, liposome composition and peroxidation temperature) were selected following characterization of bilayer physical state, and not only by evaluation of peroxidation rate. Thus, the experiments described provide a routine screening procedure that would be appropriate for assessing the activity profile of novel free-radical scavengers.
大单层囊泡（LUVs）通常被认为是过氧化研究的合适模型。在本报告中，采用二棕榈酰磷脂酰胆碱（DPPC）/亚油酸混合的LUVs作为模型膜，以验证生育酚（一种有效的代表性抗氧化剂）对2,2'-偶氮二（2-脒基丙烷）盐酸盐诱导的过氧化的抑制作用（通过监测共轭二烯积累来评估）。在该模型中，在表征双层物理状态之后选择合适的实验条件（特别是脂质体组成和过氧化温度），而不仅仅是通过评估过氧化速率。因此，所描述的实验提供了一种常规筛选程序，适用于评估新型自由基清除剂的活性概况。
