• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Shearing DNA for genomic library construction.

作者信息

Hengen P N

机构信息

National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

出版信息

Trends Biochem Sci. 1997 Jul;22(7):273-4. doi: 10.1016/s0968-0004(97)01080-3.

DOI:10.1016/s0968-0004(97)01080-3
PMID:9255070
Abstract

Methods and reagents is a unique monthly column that highlights current discussion in the newsgroup bionet.molibio.methds-reagnts, available on the internet. This month's column discusses the pros and cons of various techniques used to shear DNA for shotgun cloning. For details on how to partake in the newsgroup, see the accompanying box.

摘要

相似文献

1
Shearing DNA for genomic library construction.
Trends Biochem Sci. 1997 Jul;22(7):273-4. doi: 10.1016/s0968-0004(97)01080-3.
2
Methods and reagents. Degraded DNA and gel tornados.
Trends Biochem Sci. 1997 Apr;22(4):138-9. doi: 10.1016/s0968-0004(97)01022-0.
3
Methods and reagents. Reducing background colonies with positive selection vectors.
Trends Biochem Sci. 1997 Mar;22(3):105-6. doi: 10.1016/s0968-0004(97)01009-8.
4
Recovering DNA from agarose gels.从琼脂糖凝胶中回收DNA。
Trends Biochem Sci. 1994 Sep;19(9):388-9. doi: 10.1016/0968-0004(94)90117-1.
5
Methods and reagents. Eliminating ghost bands from plasmid preps.
Trends Biochem Sci. 1996 Nov;21(11):441-2. doi: 10.1016/s0968-0004(96)30041-8.
6
Methods and reagents. Is your fluorometer on the blink?
Trends Biochem Sci. 1996 Aug;21(8):317-8. doi: 10.1016/0968-0004(96)30031-5.
7
Purification of His-Tag fusion proteins from Escherichia coli.从大肠杆菌中纯化His标签融合蛋白。
Trends Biochem Sci. 1995 Jul;20(7):285-6. doi: 10.1016/s0968-0004(00)89045-3.
8
Wayward PCR primers.
Trends Biochem Sci. 1995 Jan;20(1):42-4. doi: 10.1016/s0968-0004(00)88950-1.
9
Reamplification of PCR fragments.
Trends Biochem Sci. 1995 Mar;20(3):124-5. doi: 10.1016/s0968-0004(00)88980-x.
10
Methods and reagents. On the magic of mini-preps...
Trends Biochem Sci. 1994 Apr;19(4):182-3. doi: 10.1016/0968-0004(94)90283-6.

引用本文的文献

1
Microfluidic Continuous Flow DNA Fragmentation based on a Vibrating Sharp-tip.基于振动尖点的微流控连续流DNA片段化
Microfluid Nanofluidics. 2022 Dec;26(12). doi: 10.1007/s10404-022-02610-7. Epub 2022 Nov 15.
2
The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation.针诱导气泡作用下台式超声水浴中DNA片段化增强的模拟与实验研究。
Biomicrofluidics. 2022 Jul 28;16(4):044103. doi: 10.1063/5.0101740. eCollection 2022 Jul.
3
Cavitation Enhancement Increases the Efficiency and Consistency of Chromatin Fragmentation from Fixed Cells for Downstream Quantitative Applications.
空化增强提高了固定细胞染色质片段化的效率和一致性，有利于下游定量应用。
Biochemistry. 2018 May 15;57(19):2756-2761. doi: 10.1021/acs.biochem.8b00075. Epub 2018 Apr 25.
4
High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System.在鼓泡系统中实现高效的流体动力 DNA 片段化。
Sci Rep. 2017 Jan 18;7:40745. doi: 10.1038/srep40745.
5
Cavitation Enhancing Nanodroplets Mediate Efficient DNA Fragmentation in a Bench Top Ultrasonic Water Bath.空化增强纳米液滴在台式超声水浴中介导高效的DNA片段化
PLoS One. 2015 Jul 17;10(7):e0133014. doi: 10.1371/journal.pone.0133014. eCollection 2015.
6
A device for automated hydrodynamic shearing of genomic DNA.一种用于基因组DNA自动水力剪切的装置。
Biotechniques. 2009 Jun;46(7):553-6. doi: 10.2144/000113123.
7
ROMA: an in vitro approach to defining target genes for transcription regulators.ROMA：一种用于确定转录调节因子靶基因的体外方法。
Methods. 2009 Jan;47(1):73-7. doi: 10.1016/j.ymeth.2008.10.009. Epub 2008 Oct 21.
8
Shear-induced assembly of lambda-phage DNA.剪切诱导的λ噬菌体DNA组装。
Biophys J. 2000 Sep;79(3):1530-6. doi: 10.1016/S0006-3495(00)76404-6.