Vatteroni M, Maggi F, Morrica A, Fornai C, Giorgi M, Pistello M, Bendinelli M
Department of Biomedicine, University of Pisa, Italy.
J Virol Methods. 1997 Jul;66(2):187-94. doi: 10.1016/s0166-0934(97)00054-2.
A panel of 61 HCV isolates belonging to five different subtypes were used to evaluate five methods for rapid typing of HCV RNA: an in-house type-specific polymerase chain reaction based on the core region (type-specific PCR), a commercial amplification of the core region followed by hybridisation to probe coated wells (DEIA), a commercial amplification of the 5'-UTR region followed by hybridisation to probes immobilised on strips (LiPA), an in-house restriction fragment polymorphism analysis of the 5'UTR (RFLP), and a commercial serological method using synthetic peptides from the NS4 region (serotyping). The correct viral type was identified in 90% of cases by DEIA, in 82% of cases by type-specific PCR, in 80% of cases by LiPA and RFLP, and in 67% of cases by serotyping. Correct identification of the virus subtype was much less frequent and was beyond the performance characteristics of some assays. Major problems were found in the identification of isolates belonging to type 2. This was probably at least partly due to the fact that all type 2 isolates in the viral panel were of subtype 2c, which has been considered rare until recently.
使用一组属于五种不同亚型的61株丙型肝炎病毒（HCV）分离株来评估五种HCV RNA快速分型方法：基于核心区域的内部型特异性聚合酶链反应（型特异性PCR）、核心区域的商业扩增随后与包被探针的孔进行杂交（DEIA）、5'-非翻译区（5'-UTR）区域的商业扩增随后与固定在条带上的探针进行杂交（LiPA）、5'UTR的内部限制性片段多态性分析（RFLP）以及使用来自NS4区域的合成肽的商业血清学方法（血清分型）。通过DEIA在90%的病例中鉴定出正确的病毒类型，通过型特异性PCR在82%的病例中鉴定出，通过LiPA和RFLP在80%的病例中鉴定出，通过血清分型在67%的病例中鉴定出。病毒亚型的正确鉴定频率要低得多，并且超出了一些检测方法的性能特征。在鉴定属于2型的分离株时发现了主要问题。这可能至少部分是由于病毒组中所有2型分离株均为2c亚型，而直到最近2c亚型一直被认为是罕见的。
