Tisminetzky S, Gerotto M, Pontisso P, Chemello L, Prescott L E, Rose K A, Baralle F, Simmonds P, Alberti A
International Center for Genetic Engineering and Biotechnology, Trieste, Italy.
J Virol Methods. 1995 Nov;55(3):303-7. doi: 10.1016/0166-0934(95)00067-x.
The usefulness of identification of hepatitis C virus (HCV) genotype has recently been investigated for the clinical management of patients infected by HCV. In the present study, the HCV genotype infecting 127 patients was determined by two different methods: HCV genotyping using a dot-blot assay with type-specific probes derived from the 5'-UTR of HCV genome and HCV serotyping using an ELISA system in which type-specific antibodies against the NS4 region were detected. Overall, a good correlation of the two methods was observed, the main discrepancy being 4 patients with sequence-confirmed HCV-2 (2 cases) and HCV-3 (2 cases) genotypes recognized as HCV-1 by serotyping. Mixed infections were not detected by either method. In 19 PCR negative sera, in which the HCV genotype could not be evaluated, no particular serotype profile was observed. In conclusion, the molecular and serological techniques are almost equivalent in determining the viral type, although in individual cases, especially in PCR negative patients, the clinical meaning of the serotyping result remains to be determined.
最近，人们对丙型肝炎病毒（HCV）基因型鉴定在HCV感染患者临床管理中的作用进行了研究。在本研究中，采用两种不同方法确定了感染127例患者的HCV基因型：一种是使用源自HCV基因组5'-UTR的型特异性探针的斑点杂交法进行HCV基因分型，另一种是使用ELISA系统进行HCV血清分型，该系统可检测针对NS4区域的型特异性抗体。总体而言，观察到两种方法具有良好的相关性，主要差异在于有4例经序列确认的HCV-2（2例）和HCV-3（2例）基因型患者，其血清分型被认定为HCV-1。两种方法均未检测到混合感染。在19份PCR阴性血清中，无法评估HCV基因型，未观察到特定的血清型谱。总之，分子技术和血清学技术在确定病毒类型方面几乎等效，尽管在个别情况下，尤其是PCR阴性患者中，血清分型结果的临床意义仍有待确定。
