Eye Hospital, University Medical Centre, 1000 Ljubljana, Slovenia.
CELLS-ALBA, Carrer de la Llum 2-26, 08290 Cerdanyola del Valles, Barcelona, Spain.
Int J Mol Sci. 2021 May 16;22(10):5249. doi: 10.3390/ijms22105249.
Ultraviolet (UV) irradiation is an important risk factor in cataractogenesis. Lens epithelial cells (LECs), which are a highly metabolically active part of the lens, play an important role in UV-induced cataractogenesis. The purpose of this study was to characterize cell compounds such as nucleic acids, proteins, and lipids in human UV C-irradiated anterior lens capsules (LCs) with LECs, as well as to compare them with the control, non-irradiated LCs of patients without cataract, by using synchrotron radiation-based Fourier transform infrared (SR-FTIR) micro-spectroscopy. In order to understand the effect of the UV C on the LC bio-macromolecules in a context of cataractogenesis, we used the SR-FTIR micro-spectroscopy setup installed on the beamline MIRAS at the Spanish synchrotron light source ALBA, where measurements were set to achieve a single-cell resolution with high spectral stability and high photon flux. UV C irradiation of LCs resulted in a significant effect on protein conformation with protein formation of intramolecular parallel β-sheet structure, lower phosphate and carboxyl bands in fatty acids and amino acids, and oxidative stress markers with significant increase of lipid peroxidation and diminishment of the asymmetric CH band.
紫外线（UV）照射是白内障形成的一个重要危险因素。晶状体上皮细胞（LEC）是晶状体中代谢非常活跃的一部分，在 UV 诱导的白内障形成中起着重要作用。本研究的目的是通过基于同步辐射的傅里叶变换红外（SR-FTIR）微光谱学，对人 UV C 照射的前晶状体囊（LC）中的细胞化合物（如核酸、蛋白质和脂质）进行特征化，并将其与白内障患者的未照射对照 LC 进行比较。为了了解在白内障形成的情况下，UV C 对 LC 生物大分子的影响，我们使用了安装在西班牙同步辐射光源 ALBA 的 MIRAS 光束线上的 SR-FTIR 微光谱学装置，该装置的测量设置实现了单细胞分辨率，具有高光谱稳定性和高光子通量。LC 的 UV C 照射对蛋白质构象产生了显著影响，形成了分子内平行β-折叠结构的蛋白质，脂肪酸和氨基酸中的磷酸和羧基带降低，以及氧化应激标志物脂质过氧化的显著增加和不对称 CH 带的减少。
