免疫刺激DNA序列作为促进辅助性T细胞1型的佐剂发挥作用。

Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants.

作者信息

Roman M, Martin-Orozco E, Goodman J S, Nguyen M D, Sato Y, Ronaghy A, Kornbluth R S, Richman D D, Carson D A, Raz E

机构信息

Dynavax Technologies Corporation, San Diego, California 92121, USA.

出版信息

Nat Med. 1997 Aug;3(8):849-54. doi: 10.1038/nm0897-849.

DOI:10.1038/nm0897-849

PMID:9256274

Abstract

An adjuvant role for certain short bacterial immunostimulatory DNA sequences (ISSs) has recently been proposed on the basis of their ability to stimulate T helper-1 (Th1) responses in gene-vaccinated animals. We report here that noncoding, ISS-enriched plasmid DNAs or ISS oligonucleotides (ISS-ODNs) potently stimulate immune responses to coadministered antigens. The ISS-DNAs suppress IgE synthesis, but promote IgG and interferon-gamma (IFN-gamma) production. They furthermore initiate the production of IFN-gamma, IFN-alpha, IFN-beta, and interleukins 12 and 18, all of which foster Th1 responses and enhance cell-mediated immunity. Consideration should be given to adding noncoding DNA adjuvants to inactivated or subunit viral vaccines that, by themselves, provide only partial protection from infection.

摘要

最近，基于某些短的细菌免疫刺激DNA序列（ISSs）能够在基因疫苗接种动物中刺激辅助性T细胞1（Th1）反应，有人提出了它们的佐剂作用。我们在此报告，富含ISS的非编码质粒DNA或ISS寡核苷酸（ISS-ODNs）能有效刺激对共同给药抗原的免疫反应。ISS-DNAs抑制IgE合成，但促进IgG和干扰素-γ（IFN-γ）产生。它们还能启动IFN-γ、IFN-α、IFN-β以及白细胞介素12和18的产生，所有这些都能促进Th1反应并增强细胞介导的免疫。对于仅能提供部分抗感染保护的灭活或亚单位病毒疫苗，应考虑添加非编码DNA佐剂。