Miura H, Yanazawa M, Kato K, Kitamura K
Mitsubishi Kasei Institute of Life Sciences, Machida-shi, Tokyo, Japan.
Mech Dev. 1997 Jul;65(1-2):99-109. doi: 10.1016/s0925-4773(97)00062-2.
We have isolated a novel homeobox gene that is expressed in the vertebrate central nervous system and which shows striking similarity to the Drosophila al gene in the homeodomain (85% identity) and in a 17 amino acid-sequence near the carboxyl-terminus. This gene was designated Arx (aristaless related homeobox gene) in consideration of its structural similarity to the al gene. Arx was highly conserved between mouse and zebrafish. Neuromeric expression in the forebrain and longitudinal expression in the floor plate were observed in mouse and zebrafish. The expression of Arx in the ganglionic eminence and ventral thalamus overlapped regionally with that of Dlx1, but the cell layer where Arx is expressed differed from that of the Dlx1. This gene was also found to be expressed in the dorsal telencephalon (presumptive cerebral cortex) of mouse embryos. The structure and expression pattern of Arx with respect to any possible relationship to al and Dlx1, as well as the function of Arx in the floor plate are discussed.
我们分离出了一个新的同源异型盒基因，该基因在脊椎动物中枢神经系统中表达，并且在同源结构域与果蝇al基因表现出显著相似性（85%的一致性），在羧基末端附近的一段17个氨基酸的序列中也有相似性。考虑到该基因与al基因的结构相似性，将其命名为Arx（无触角相关同源异型盒基因）。Arx在小鼠和斑马鱼之间高度保守。在小鼠和斑马鱼中均观察到前脑的神经节段性表达以及底板的纵向表达。Arx在神经节隆起和腹侧丘脑重叠区域的表达与Dlx1的表达在区域上有重叠，但Arx的表达细胞层与Dlx1不同。还发现该基因在小鼠胚胎的背侧端脑（推测的大脑皮层）中表达。本文讨论了Arx的结构和表达模式，及其与al和Dlx1的任何可能关系，以及Arx在底板中的功能。
