Mukaida T, Ichiba S, Aoe M, Yamashita M, Okabe K, Date H, Andou A, Shimizu N
Department of Surgery II, Okayama University Medical School, Japan.
Nihon Kyobu Geka Gakkai Zasshi. 1997 Jul;45(7):964-9.
In this study, we investigated whether the regenerated epithelia were recipient phenotype or donor phenotype using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method. Preoperatively 24 mongrel dogs were classified to 14 types by PCR-RFLP result from peripheral blood. The PCR-RFLP result of peripheral blood agreed to that of recipient epithelia. The cryopreserved tracheal allotransplantation was performed among the five pairs in which we could distinguish donor from recipient by PCR-RFLP. The epithelia of graft at 10 days after transplantation showed donor phenotype, but the epithelia at postoperative 20 days or more showed recipient phenotype. These results showed that allogenic epithelium remained in early post-transplant time and was gradually omitted. The epithelia migrated gradually from the anastomotic site, and the graft was covered with regenerated epithelia showing recipient phenotype within about 50 post-transplant days.
在本研究中，我们采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，研究再生上皮是受体表型还是供体表型。术前，根据外周血的PCR-RFLP结果，将24只杂种犬分为14种类型。外周血的PCR-RFLP结果与受体上皮的结果一致。在通过PCR-RFLP能够区分供体和受体的五对犬中进行了冷冻保存的气管同种异体移植。移植后10天移植物的上皮显示供体表型，但术后20天及更长时间的上皮显示受体表型。这些结果表明，同种异体上皮在移植后早期留存，并逐渐被清除。上皮从吻合部位逐渐迁移，在移植后约50天内，移植物被显示受体表型的再生上皮覆盖。
