Brooks D E, Garcia G A, Dreyer E B, Zurakowski D, Franco-Bourland R E
Department of Small and Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville 32610, USA.
Am J Vet Res. 1997 Aug;58(8):864-7.
To analyze the vitreal amino acid concentrations in dogs with breed-related primary glaucoma to determine whether excitotoxic amino acids associated with retinal genglion cell death in other species were present in affected dogs.
11 normal control and 10 glaucomatous canine eyes.
Amino acid analyses were performed by high-pressure liquid chromatography in masked manner.
Eyes from dogs with primary glaucoma had significantly high vitreal glutamate concentration, compared with values for eyes of clinically normal control dogs. Mean (+/-SD) glutamate concentrations were 31.7 +/- 12.4 and 6.9 +/- 6.3 microM in glaucomatous and normal eyes, respectively (P < 0.0001). Eyes from dogs with glaucoma also had lower vitreal glycine (37.0 +/- 17.0 vs 59.4 +/- 28.2 microM; P < 0.043) and higher of vitreal tryptophan (39.0 +/- 22.8 vs 17.5 +/- 11.2 microM; P < 0.012) concentrations, compared with values for normal eyes.
Glutamate concentration potentially toxic to retinal ganglion cells is associated with the pathogenesis of primary glaucoma in dogs. Increased glutamate concentration provides evidence of an ischemic mechanism for retinal ganglion cell death and optic nerve atrophy in dogs with glaucoma.
The emphasis on reduction and normalization of high intraocular pressure as the primary focus of treatment for glaucoma in dogs should be augmented by other therapeutic approaches.
分析与品种相关的原发性青光眼犬的玻璃体内氨基酸浓度，以确定受影响犬眼中是否存在与其他物种视网膜神经节细胞死亡相关的兴奋性毒性氨基酸。
11只正常对照犬眼和10只青光眼犬眼。
采用高压液相色谱法对氨基酸进行盲法分析。
与临床正常对照犬的眼相比，原发性青光眼犬的眼玻璃体内谷氨酸浓度显著升高。青光眼眼和正常眼的谷氨酸平均（±标准差）浓度分别为31.7±12.4和6.9±6.3微摩尔/升（P<0.0001）。与正常眼相比，青光眼犬的眼玻璃体内甘氨酸浓度也较低（37.0±17.0对59.4±28.2微摩尔/升；P<0.043），色氨酸浓度较高（39.0±22.8对17.5±11.2微摩尔/升；P<0.012）。
对视网膜神经节细胞具有潜在毒性的谷氨酸浓度与犬原发性青光眼的发病机制有关。谷氨酸浓度升高为青光眼犬视网膜神经节细胞死亡和视神经萎缩的缺血机制提供了证据。
犬青光眼治疗以降低和使高眼压正常化为主要重点，应辅以其他治疗方法。
