通过毛细管电泳激光诱导荧光检测法检测维多利亚多管水母绿色荧光蛋白。
Detection of Aequorea victoria green fluorescent protein by capillary electrophoresis laser induced fluorescence detection.
作者信息
Craig D B, Wong J C, Dovichi N J
机构信息
Department of Chemistry, University of Alberta, Edmonton, Canada.
出版信息
Biomed Chromatogr. 1997 Jul-Aug;11(4):205-6. doi: 10.1002/(SICI)1099-0801(199707)11:4<205::AID-BMC675>3.0.CO;2-#.
Abstract
Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laser-induced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 x 10(-12) M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.
摘要
利用鞘流池中的毛细管后激光诱导荧光检测，通过毛细管电泳法对维多利亚多管水母绿色荧光蛋白进行了测定。在进样体积为17纳升时，检测限为3.0×10⁻¹² M蛋白质，相当于3100个分子的质量。
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