Craig D B, Wong J C, Dovichi N J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Biomed Chromatogr. 1997 Jul-Aug;11(4):205-6. doi: 10.1002/(SICI)1099-0801(199707)11:4<205::AID-BMC675>3.0.CO;2-#.
Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laser-induced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 x 10(-12) M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.
利用鞘流池中的毛细管后激光诱导荧光检测,通过毛细管电泳法对维多利亚多管水母绿色荧光蛋白进行了测定。在进样体积为17纳升时,检测限为3.0×10⁻¹² M蛋白质,相当于3100个分子的质量。