Sobhani Kimia, Michels David A, Dovichi Norman J
Department of Chemistry, University of Washington, Box 351700, Seattle, Washington 98195-1700, USA.
Appl Spectrosc. 2007 Jul;61(7):777-9. doi: 10.1366/000370207781393208.
The sheath-flow cuvette is a key component in a high-sensitivity post-column laser-induced fluorescence detector for capillary electrophoresis. Most designs are based on commercial cuvettes originally manufactured for use in a flow cytometer. In these devices, a quartz flow chamber is held in a stainless-steel fixture that is difficult to machine and subjects the cuvette to a torque when sealed, which frequently leads to damage of the flow chamber. In this report we present a design for a cuvette that may easily be constructed. This design uses compression to hold and seal the quartz flow chamber without applying torque. The system produces detection limits (3sigma) of 115 yoctomoles (70 copies) for FQ-labeled carbonic anhydrase.
鞘流比色皿是毛细管电泳高灵敏度柱后激光诱导荧光检测器的关键部件。大多数设计基于最初为流式细胞仪制造的商用比色皿。在这些装置中，石英流动室被固定在一个难以加工的不锈钢夹具中，密封时会使比色皿受到扭矩作用，这经常导致流动室损坏。在本报告中，我们提出了一种易于构建的比色皿设计。该设计利用压缩来固定和密封石英流动室，而不施加扭矩。该系统对FQ标记的碳酸酐酶的检测限（3σ）为115 yoctomoles（70个拷贝）。
