Cleary J, Mohr D, Adams M R, Celermajer D S, Stocker R
Biochemistry Unit, Royal Prince Alfred Hospital, Sydney NSW, Australia.
Free Radic Res. 1997 Feb;26(2):175-82. doi: 10.3109/10715769709097796.
Oxidative modification of low-density lipoprotein (LDL), regarded an early event in atherogenesis, is associated with the depletion of the lipoprotein's antioxidants. We tested whether the levels of major lipophilic antioxidants in the blood of patients with advanced atherosclerosis are different to those in age-matched controls. On average, plasma ubiquinol-10, total coenzyme Q and coenzyme Q redox status were slightly lower whereas the levels of alpha-tocopherol were slightly higher in patients (63 +/- 11 years, n = 32) than controls (64 +/- 10 years, n = 24). However, these differences were not statistically significant (p > 0.05). The levels of antioxidants in LDL isolated from a subset of patients (n = 20) and controls (n = 15) were also indifferent, and hydroperoxides of cholesteryl esters were undetectable (detection limit 10 nM) in plasma of patients (n = 11) and controls (n = 10). The data suggests that plasma and LDL levels of lipophilic antioxidants are not depleted in patients suffering from severe atherosclerosis, and that neither parameter serves as a useful diagnostic indicator for this disease.
低密度脂蛋白（LDL）的氧化修饰被认为是动脉粥样硬化发生的早期事件，与脂蛋白抗氧化剂的消耗有关。我们测试了晚期动脉粥样硬化患者血液中主要亲脂性抗氧化剂的水平是否与年龄匹配的对照组不同。平均而言，患者（63±11岁，n = 32）的血浆泛醇-10、总辅酶Q和辅酶Q氧化还原状态略低于对照组（64±10岁，n = 24），而α-生育酚水平略高于对照组。然而，这些差异无统计学意义（p>0.05）。从一部分患者（n = 20）和对照组（n = 15）中分离出的LDL中的抗氧化剂水平也无差异，并且在患者（n = 11）和对照组（n = 10）的血浆中未检测到胆固醇酯的氢过氧化物（检测限10 nM）。数据表明，患有严重动脉粥样硬化的患者血浆和LDL中的亲脂性抗氧化剂水平并未降低，且这两个参数均不能作为该疾病的有用诊断指标。
