Aejmelaeus R, Metsä-Ketelä T, Laippala P, Solakivi T, Alho H
Department of Pharmacology, Clinical Pharmacology and Toxicology, Medical School, University of Tampere, Finland.
Mol Aspects Med. 1997;18 Suppl:S113-20. doi: 10.1016/s0098-2997(97)00002-2.
Evidence is rapidly accumulating that oxidative modification of low density lipoprotein (LDL) may play an important role in the pathogenesis of atherosclerosis. In this study we measured the total peroxyl radical trapping capacity of human plasma LDL phospholipids (TRAPLDL) with a luminescent method. The study was carried out with 70 healthy volunteers, aged 28-77. In males an age-related decrease in TRAPLDL was observed. In the age group under 50 years the mean TRAPLDL was 31.36 +/- 1.45 pmol peroxyl radicals/nmol Pi; among those over 50 years it was significantly lower at 26.67 0.94 pmol/nmol Pi. As regards the components of TRAPLDL, the concentration of LDL-ubiquinol did not change and a non-significant decrease in the LDL-tocopherol concentration was detected with age. In females, the mean TRAPLDL, LDL-ubiquinol-10 and tocopherol concentrations did not differ between the age groups. When 17 of the participants were given coenzyme Q10 (Q10) supplementation, 100 mg/day, a highly significant increase in LDL-ubiquinol concentration was detected. Our results indicate that LDL antioxidant defenses tend to decrease with age in the Finnish male population. The decline is most significant in males under 50 years; in older age groups the values remain stable at a low level. Q10 supplementation doubles the number of ubiquinol-10-containing LDL molecules and may therefore have an inhibitory effect on LDL oxidation.
越来越多的证据表明，低密度脂蛋白（LDL）的氧化修饰可能在动脉粥样硬化的发病机制中起重要作用。在本研究中，我们采用发光法测量了人血浆LDL磷脂的总过氧自由基捕获能力（TRAPLDL）。该研究对70名年龄在28 - 77岁的健康志愿者进行。在男性中观察到TRAPLDL随年龄下降。在50岁以下年龄组中，平均TRAPLDL为31.36±1.45 pmol过氧自由基/nmol Pi；在50岁以上人群中，该值显著降低，为26.67±0.94 pmol/nmol Pi。关于TRAPLDL的组成成分，LDL - 泛醇的浓度没有变化，并且随着年龄增长，LDL - 生育酚浓度有不显著的下降。在女性中，各年龄组的平均TRAPLDL、LDL - 泛醇 - 10和生育酚浓度没有差异。当17名参与者每天补充100 mg辅酶Q10（Q10）时，检测到LDL - 泛醇浓度显著升高。我们的结果表明，在芬兰男性人群中，LDL抗氧化防御能力倾向于随年龄下降。这种下降在50岁以下男性中最为显著；在老年组中，该值在低水平保持稳定。补充Q10可使含泛醇 - 10的LDL分子数量增加一倍，因此可能对LDL氧化有抑制作用。
