Poonwan N, Mikami Y, Poosuwan S, Boon-Long J, Mekha N, Kusum M, Yazawa K, Tanaka R, Nishimura K, Konyama K
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Eur J Epidemiol. 1997 Apr;13(3):335-40. doi: 10.1023/a:1007376917836.
One hundred and thirty-nine strains of Cryptococcus neoformans were isolated in Thailand from clinical specimens including 97 AIDS patients: 67 from Northern, 48 from Central, 17 from Northeastern and 7 from Southern regional hospitals. Six out of the 139 strains were serotype B and the remaining 133 were A. There was no correlation between serotypes and regional distribution. To our knowledge, this is the first report of serotyping studies on C. neoformans in Thailand. Studies on random amplified polymorphic DNA (RAPD) analysis showed that this method is useful for the differentiation of C. neoformans var. gattii (serotypes B and C) and C. neoformans var. neoformans (serotypes A and D). They also indicated that Thai isolates of C. neoformans var. gattii (serotype B) were a homogeneous group on the basis of their genotypes. Antifungal susceptibility tests using 5 antifungal agents including amphotericin B, fluconazole, flucytosine, itraconazole and micronazole against 50 selected strains of C. neoformans showed that they were sensitive to all of the antifungal agents tested except for one strain that was resistant to flucytosine.
在泰国，从包括97例艾滋病患者在内的临床标本中分离出139株新型隐球菌：67株来自北部地区医院，48株来自中部地区医院，17株来自东北地区医院，7株来自南部地区医院。139株菌株中6株为B血清型，其余133株为A血清型。血清型与地区分布之间无相关性。据我们所知，这是泰国关于新型隐球菌血清分型研究的首次报告。随机扩增多态性DNA（RAPD）分析研究表明，该方法可用于区分新型隐球菌变种加蒂变种（血清型B和C）和新型隐球菌变种新型隐球菌变种（血清型A和D）。研究还表明，泰国分离的新型隐球菌变种加蒂变种（血清型B）基于其基因型属于一个同质群体。使用包括两性霉素B、氟康唑、氟胞嘧啶、伊曲康唑和咪康唑在内的5种抗真菌药物对50株选定的新型隐球菌菌株进行的抗真菌药敏试验表明，除1株对氟胞嘧啶耐药外，它们对所有测试的抗真菌药物均敏感。
