Mikami Y, Sakamoto T, Yazawa K, Gonoi T, Ueno Y, Hasegawa S
Division of Chemotherapy, Chiba University, Japan.
Mycoses. 1994 Jan-Feb;37(1-2):27-33. doi: 10.1111/j.1439-0507.1994.tb00281.x.
The in vitro antifungal activities of itraconazole and its active hydroxyl metabolite, hydroxy-itraconazole (R 63372), against Aspergillus fumigatus, Candida albicans and Cryptococcus neoformans were compared by visual assessment of growth as well as by colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay using microtitre plates containing four different media. Minimum inhibitory concentration (MIC) end points determined by the colorimetric MTT assay correlated well with those obtained by visual assay. The two drugs showed different MIC values depending on the medium used. The activity of itraconazole was equal to or greater than the activity of hydroxy-itraconazole against most of the fungi tested. Both drugs showed lower MIC values against A. fumigatus and Cr. neoformans in brain heart infusion broth (BHI) medium than in yeast nitrogen base (YNBG) medium, Sabouraud glucose broth (SAB) or "synthetic amino acid medium, fungal" (SAAMF). However, the MIC end point of these drugs against C. albicans in BHI and SAB media was difficult to determine visually as well as by MTT assay. In C. albicans, the MTT assay method using SAAMF and YNBG media is recommended for the determination of MICs.
通过肉眼观察生长情况以及使用含有四种不同培养基的微量滴定板进行比色MTT[3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H四氮唑溴盐]测定，比较了伊曲康唑及其活性羟基代谢物羟基伊曲康唑（R 63372）对烟曲霉、白色念珠菌和新型隐球菌的体外抗真菌活性。比色MTT测定法确定的最低抑菌浓度（MIC）终点与肉眼观察法获得的结果相关性良好。根据所用培养基的不同，这两种药物显示出不同的MIC值。在大多数测试真菌中，伊曲康唑的活性等于或大于羟基伊曲康唑的活性。在脑心浸液肉汤（BHI）培养基中，这两种药物对烟曲霉和新型隐球菌的MIC值均低于酵母氮源（YNBG）培养基、沙氏葡萄糖肉汤（SAB）或“合成氨基酸培养基，真菌专用”（SAAMF）中的MIC值。然而，通过肉眼观察以及MTT测定法很难确定这两种药物在BHI和SAB培养基中对白色念珠菌的MIC终点。对于白色念珠菌，推荐使用SAAMF和YNBG培养基的MTT测定法来确定MIC值。
