Choi B O, Yamaki T, Tatewaki K, Ibayashi Y, Hashi K
Department of Neurosurgery, Sapporo Medical University School of Medicine, Hokkaido, Japan.
J Neurooncol. 1997 Sep;34(3):211-9. doi: 10.1023/a:1005742716197.
Glycolipid compositions of the human glioma cell line T98G were studied during each phase of the cell cycle to see if those cell surface molecules are concerned with cell proliferation. In vitro cultured non-synchronized T98G cells are composed of ceramidemonohexoside (CMH), ceramidedihexoside (CDH), ceramidetrihexoside (CTH) and neolactotetraosylceramide (nLc4Cer) as neutral glycolipids, and of sulfatide (CS), gangliosides GM3, GM2, GD1a and several other gangliosides as acidic ones. While total glycolipid content per cellular weight was shown to be increased during the M phase, deletion of complex gangliosides particularly b-series gangliosides was recognized (p < 0.05). The glycolipid profile in other phases was fairly consistent, and there was no glycolipid molecule specific to a certain phase of the cell cycle. Relative enhancement of simple gangliosides with a decrease of complex ones during mitotic division may imply the functional involvement of complex gangliosides in cell-cell or cell-matrix attachment, which may have to be abandoned during the process of detachment from the matrix or cellular cleavage.
研究了人胶质瘤细胞系T98G在细胞周期各阶段的糖脂组成，以观察这些细胞表面分子是否与细胞增殖有关。体外培养的未同步化T98G细胞含有神经酰胺单己糖苷（CMH）、神经酰胺二己糖苷（CDH）、神经酰胺三己糖苷（CTH）和新乳糖四糖神经酰胺（nLc4Cer）作为中性糖脂，以及硫苷脂（CS）、神经节苷脂GM3、GM2、GD1a和其他几种神经节苷脂作为酸性糖脂。虽然每细胞重量的总糖脂含量在M期有所增加，但发现复合神经节苷脂尤其是b系列神经节苷脂减少（p < 0.05）。其他阶段的糖脂谱相当一致，并且没有特定于细胞周期某一阶段的糖脂分子。在有丝分裂期间简单神经节苷脂相对增加而复合神经节苷脂减少，这可能意味着复合神经节苷脂在细胞 - 细胞或细胞 - 基质附着中起作用，而在从基质脱离或细胞分裂过程中可能必须放弃这种附着。
