Pedersen P V, Crooks M J, Brown K F
J Pharm Sci. 1977 Oct;66(10):1458-61. doi: 10.1002/jps.2600661028.
A new method of treating dynamic dialysis data to obtain binding parameters for drug-macromolecule interactions is presented. This method allows the determination of binding parameters directly from dialysis data according to a theoretical model. It is not necessary to determine the dialysis rate constant accurately in a separate experiment, and bias is not introduced due to differentiation. The proposed method should be applicable where the drug is substantially bound to the dialysis membrane.
本文提出了一种处理动态透析数据以获取药物 - 大分子相互作用结合参数的新方法。该方法可根据理论模型直接从透析数据中确定结合参数。无需在单独的实验中精确测定透析速率常数，且不会因微分而引入偏差。所提出的方法应适用于药物与透析膜大量结合的情况。
