Garrett N E, Cruwys S C, Kidd B L, Tomlinson D R
Department of Pharmacology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK.
Neurosci Lett. 1997 Jul 11;230(1):5-8. doi: 10.1016/s0304-3940(97)00458-8.
We have investigated the effect of capsaicin pretreatment (50 mg kg(-1) s.c.) on substance P, preprotachykinin (PPT) mRNA, and nerve growth factor (NGF), plus its high-affinity receptor, trkA, in adult rats with adjuvant arthritis. Twenty one days after induction of adjuvant arthritis, sciatic nerve levels of substance P were significantly increased whilst there was a small but non-significant increase in gamma-PPT mRNA and substance P in L4/L5 dorsal root ganglia (DRG). NGF levels in sciatic nerve and foot skin as well as DRG trkA mRNA were unaltered after 21 days arthritis suggesting that NGF may not play a role in chronic inflammation. Capsaicin treatment of naive rats significantly reduced substance P in all tissues and NGF levels in the sciatic nerve. In contrast, gamma-PPT mRNA and trkA mRNA expression in DRG were significantly increased after capsaicin treatment. The nervous and skin tissues used in this study were harvested from the same rats in which we had previously shown that capsaicin pretreatment significantly attenuated the severity of arthritis (Cruwys, S.C., Garrett, N.E. and Kidd, B.L., Sensory denervation with capsaicin attenuates inflammation and nociception in arthritic rats, Neurosci. Lett., 193 (1995) 205-207). Arthritis in capsaicin-treated rats had no effect on substance P or NGF levels in any tissue when compared with capsaicin-treated control rats, suggesting that pharmacological impairment of the sensory nervous system can reduce the severity of inflammatory joint disease.
我们研究了辣椒素预处理（50毫克/千克，皮下注射）对佐剂性关节炎成年大鼠中P物质、前速激肽原（PPT）mRNA、神经生长因子（NGF）及其高亲和力受体trkA的影响。在诱导佐剂性关节炎21天后，坐骨神经中P物质水平显著升高，而L4/L5背根神经节（DRG）中的γ-PPT mRNA和P物质有小幅但不显著的增加。关节炎21天后，坐骨神经和足部皮肤中的NGF水平以及DRG中的trkA mRNA未发生改变，这表明NGF可能在慢性炎症中不起作用。对未患关节炎的大鼠进行辣椒素处理可显著降低所有组织中的P物质以及坐骨神经中的NGF水平。相比之下，辣椒素处理后DRG中的γ-PPT mRNA和trkA mRNA表达显著增加。本研究中使用的神经和皮肤组织取自我们之前已证明辣椒素预处理可显著减轻关节炎严重程度的同一只大鼠（Cruwys, S.C., Garrett, N.E.和Kidd, B.L.，辣椒素感觉去神经支配减轻关节炎大鼠的炎症和伤害感受，《神经科学快报》，193 (1995) 205 - 207）。与辣椒素处理的对照大鼠相比，辣椒素处理的关节炎大鼠的任何组织中的P物质或NGF水平均未受关节炎影响，这表明感觉神经系统的药理学损伤可减轻炎症性关节疾病的严重程度。
