Schmelz M, Luz O, Averbeck B, Bickel A
Department of Physiology and Experimental Pathophysiology, University Erlangen/Nürnberg, Erlangen, Germany.
Neurosci Lett. 1997 Jul 18;230(2):117-20. doi: 10.1016/s0304-3940(97)00494-1.
Time course of plasma extravasation in neurogenic inflammation elicited by histamine iontophoresis and topical capsaicin application was measured in vivo by a modified intradermal microdialysis technique in humans. Single linear plasmapheresis fibers were inserted intracutaneously at the volar forearm and perfused with Ringer's solution (4 microl/min) with one microdialysis fiber located at the planned stimulation site and a second inside the axon reflex area. Protein concentration in the dialysate increased by 300% in the histamine wheal but no increase was found in the surrounding flare area. There was no protein extravasation in the skin treated with capsaicin nor in the surrounding flare region. Inside the histamine-induced flare reaction a significant increase of calcitonin gene related peptide (CGRP) was found, whereas the increase of substance P (SP) was not significant.
采用改良的皮内微透析技术，在人体中对组胺离子电渗疗法和局部应用辣椒素引发的神经源性炎症中血浆外渗的时程进行了体内测量。将单根线性血浆分离纤维经皮插入掌侧前臂，并以林格氏液（4微升/分钟）灌注，其中一根微透析纤维位于计划的刺激部位，另一根位于轴突反射区域内。组胺风团处透析液中的蛋白质浓度增加了300%，但在周围的红斑区域未发现增加。用辣椒素处理的皮肤及其周围的红斑区域均未出现蛋白质外渗。在组胺诱导的红斑反应中，发现降钙素基因相关肽（CGRP）显著增加，而P物质（SP）的增加不显著。
