二甲基亚砜与球状蛋白质的结合：一项核磁共振弛豫色散研究。
Dimethyl sulfoxide binding to globular proteins: a nuclear magnetic relaxation dispersion study.
作者信息
Jóhannesson H, Denisov V P, Halle B
机构信息
Condensed Master Magnetic Resonance Group, Department of Chemistry, Lund University, Sweden.
出版信息
Protein Sci. 1997 Aug;6(8):1756-63. doi: 10.1002/pro.5560060816.
Abstract
The 2H magnetic relaxation dispersion (NMRD) technique was used to characterize interactions of dimethyl sulfoxide (DMSO) with globular proteins. A difference NMRD experiment involving the N-acetylglucosamine trisaccharide inhibitor, demonstrated that the DMSO 2H NMRD profile in lysozyme solution is due to a single DMSO molecule bound in the active cleft, with a molecular order parameter of 0.47 +/- 0.05 and a residence time in the range 10 ns to 5 ms. With the aid of transverse 2H relaxation data, the upper bound of the residence time was further reduced to 100 microns. A 1H shift titration experiment was also performed, yielding a binding constant of 2.3 +/- 0.3 M-1 at 27 degrees C. In contrast to lysozyme, no DMSO dispersion was observed for bovine pancreatic trypsin inhibitor (BPTI), indicating that a stable DMSO-protein complex requires a cleft of appropriate geometry in addition to hydrogen-bond and hydrophobic interactions.
摘要
采用2H磁弛豫色散（NMRD）技术来表征二甲基亚砜（DMSO）与球状蛋白质的相互作用。一项涉及N-乙酰葡糖胺三糖抑制剂的差异NMRD实验表明，溶菌酶溶液中的DMSO 2H NMRD谱是由于单个DMSO分子结合在活性裂隙中，分子序参数为0.47±0.05，停留时间在10 ns至5 ms范围内。借助横向2H弛豫数据，停留时间的上限进一步降低至100微秒。还进行了1H位移滴定实验，在27℃下得到的结合常数为2.3±0.3 M-1。与溶菌酶不同，未观察到牛胰蛋白酶抑制剂（BPTI）的DMSO色散，这表明除了氢键和疏水相互作用外，稳定的DMSO-蛋白质复合物还需要具有合适几何形状的裂隙。
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