Kurokawa M, Sakagami H, Kokubu F, Noda H, Takeda M, Adachi M
First Department of Internal Medicine, School of Medicine, Showa University, Tokyo, Japan.
J Cancer Res Clin Oncol. 1997;123(7):370-6. doi: 10.1007/BF01240119.
Exposure of human leukemic cell lines (HL-60, ML-1, U-937, MOLT-4, EOL-1) to short direct-current (d.c.) treatment induced apoptotic cell death, characterized by cell shrinkage and nuclear and internucleosomal DNA fragmentation. On the other hand, human peripheral blood lymphocytes and polymorphonuclear cells were relatively resistant to d.c. treatment, and did not show any clear nuclear and DNA fragmentation. The effect of d.c. was slightly reduced by calcium depletion, but was not significantly affected by catalase or by superoxide dismutase. The present data suggest that previously reported tumor regression activities of d.c. treatment might be due, at least in part, to its apoptosis-inducing activity.
将人白血病细胞系（HL-60、ML-1、U-937、MOLT-4、EOL-1）暴露于短时间直流电处理会诱导凋亡性细胞死亡，其特征为细胞皱缩以及核和核小体间DNA片段化。另一方面，人外周血淋巴细胞和多形核细胞对直流电处理相对抗性较强，且未显示出任何明显的核和DNA片段化。钙离子耗竭会使直流电的作用略有降低，但过氧化氢酶或超氧化物歧化酶对其无显著影响。目前的数据表明，先前报道的直流电处理的肿瘤消退活性可能至少部分归因于其诱导凋亡的活性。
