Amirghofran Zahra, Bahmani Masoud, Azadmehr Abbas, Javidnia Katayoun
Immunology Department, Medical School, Shiraz University of Medical Science, 71345-1798 Shiraz, Iran.
J Cancer Res Clin Oncol. 2006 Jul;132(7):427-32. doi: 10.1007/s00432-006-0084-x. Epub 2006 Feb 14.
In the present study two medicinal herbs Linum persicum and Euphorbia cheiradenia that are native to Iran were tested for their possible anticancer effect and induction of apoptosis on human tumor cell lines including leukemia cell lines.
The effect of methanolic extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. K562 and Jurkat cell lines treated with the extracts were analyzed for the induction of apoptosis by flow cytometry using propidium iodide staining. DNA fragmentation analysis was performed.
Various concentrations of L. persicum and E. cheiradenia showed inhibitory effects on different cell lines. Two leukemic lines including K562 and Jurkat were the most sensitive cells for L. persicum with IC50 of 0.1 and 10 mug/ml, respectively. In the cultures of tumor cell lines treated with E. cheiradenia, the main inhibitory effects was for Jurkat cells with IC50 of 12.5 microg/ml. Results indicated a dose-dependent accumulation of cells in the sub-G1 phase. Study of internucleosomal DNA fragmentation showed a typical DNA laddering in agarose gels for both extracts.
The present study showed cytotoxic activity of both herbs on tumor cell lines and suggests that this effect may in part be due to the induction of apoptosis in leukemic cells.
在本研究中，对原产于伊朗的两种草药——波斯亚麻和大戟科的一种植物进行了测试，以研究它们对包括白血病细胞系在内的人类肿瘤细胞系的潜在抗癌作用和诱导凋亡的能力。
通过MTT比色法评估草药甲醇提取物对细胞增殖的抑制作用。使用碘化丙啶染色，通过流式细胞术分析用提取物处理的K562和Jurkat细胞系的凋亡诱导情况。进行DNA片段化分析。
不同浓度的波斯亚麻和大戟科的一种植物对不同细胞系均显示出抑制作用。包括K562和Jurkat在内的两种白血病细胞系对波斯亚麻最为敏感，IC50分别为0.1和10微克/毫升。在用大戟科的一种植物处理的肿瘤细胞系培养物中，主要抑制作用针对Jurkat细胞，IC50为12.5微克/毫升。结果表明细胞在亚G1期呈剂量依赖性积累。对核小体间DNA片段化的研究显示，两种提取物在琼脂糖凝胶中均呈现典型的DNA梯状条带。
本研究表明这两种草药对肿瘤细胞系均具有细胞毒性活性，并表明这种作用可能部分归因于白血病细胞凋亡的诱导。
