5,6-亚苄基-L-抗坏血酸钠及其相关化合物对人髓系白血病细胞系DNA片段化的诱导作用
Induction of DNA fragmentation in human myelogenous leukemic cell lines by sodium 5,6-benzylidene-L-ascorbate and its related compounds.
作者信息
Kuribayashi N, Sakagami H, Sakagami T, Niimi E, Shiokawa D, Ikekita M, Takeda M, Tanuma S
机构信息
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
出版信息
Anticancer Res. 1994 May-Jun;14(3A):969-76.
High-performance liquid chromatography revealed that sodium 5,6-benzylidene-L-ascorbate (SBA), dissolved in distilled water, was gradually decomposed into ascorbic acid and benzaldehyde. Among these three compounds, ascorbic acid showed the most potent cytotoxic activity. The cytotoxic activity of each compound was significantly reduced during degradation in culture medium. Agarose gel electrophoresis and fluorometric determination of DNA revealed that ascorbic acid, as well as SBA, induced DNA fragmentation into nucleosomal oligomers in human myelogenous leukemic cell lines, but not in freshly isolated human peripheral blood cells. The results suggest that antitumor activity of SBA might be at least in part mediated by the action of ascorbic acid, a degradation product of SBA.
高效液相色谱分析显示，溶解于蒸馏水中的5,6-亚苄基-L-抗坏血酸钠（SBA）会逐渐分解为抗坏血酸和苯甲醛。在这三种化合物中，抗坏血酸表现出最强的细胞毒性活性。在培养基中降解过程中，每种化合物的细胞毒性活性均显著降低。琼脂糖凝胶电泳和DNA荧光测定表明，抗坏血酸以及SBA可诱导人髓性白血病细胞系中的DNA断裂成核小体寡聚体，但在新鲜分离的人外周血细胞中则不会。结果表明，SBA的抗肿瘤活性可能至少部分是由其降解产物抗坏血酸的作用介导的。
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